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Development of an automated on-line pre-column derivatization procedure for sensitive determination of histamine in food with high-performance liquid chromatography-fluorescence detection
Peng, Jin-feng; Fang, Ke-teng; Me, Dong-hua; Ding, Bin; Yin, Ju-Yi; Cui, Xiao-mei; Zhang, Ying; Liu, Jing-fu
2008-10-31
Source PublicationJOURNAL OF CHROMATOGRAPHY A
Volume1209Issue:1Pages:70-75
AbstractAn improved sensitive method was developed and validated for the determination of histamine in food samples by using automated on-line pre-column derivatization coupled with high performance liquid chromatography and fluorescence detection (HPLC-FLD). o-Phthaldialdehyde (OPA) was adopted as derivatization reagent, and a "sandwich" (OPA+ histamine + OPA) aspiration mode for the automated online pre-column derivatization was found to efficiently enhance the method sensitivity and precision. Histamine in food samples was efficiently extracted with a methanol-phosphate buffer solution (50:50, v/v) at 60 degrees C for 30 min, and purified with Waters Oasis MCX solid-phase extraction (SPE) cartridge. The limit of quantification for this method is 0.2 mg/kg, which is very sensitive for histamine determination. With the "sandwich" injection program, 3.7% of relative standard deviation (RSD) was achieved by five replicative determinations of a sample blank spiked with 0.25 mg/kg histamine standard. Histamine in food samples such as fumitory skipjack and mackerel was analyzed with relative recoveries over 95% at spiking level of 150 mg/kg, as well as canned tuna fish and cheese with relative recoveries up to 98% at spiking levels of 0.50 and 5.0 mg/kg, respectively. The proposed method was validated with a sample from the Food Analysis Performance Assessment Scheme (FAPAS) as a standard certified material; and the results (140 +/- 6 mg/kg) agreed well with the assigned value (139 mg/kg). (C) 2008 Elsevier B.V. All rights reserved.
Department环境化学与生态毒理学国家重点实验室
KeywordHistamine Automated On-line Pre-column Derivatization Hplc-fld Food
WOS IDWOS:000260941500010
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Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/21936
Collection环境化学与生态毒理学国家重点实验室
Recommended Citation
GB/T 7714
Peng, Jin-feng,Fang, Ke-teng,Me, Dong-hua,et al. Development of an automated on-line pre-column derivatization procedure for sensitive determination of histamine in food with high-performance liquid chromatography-fluorescence detection[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1209(1):70-75.
APA Peng, Jin-feng.,Fang, Ke-teng.,Me, Dong-hua.,Ding, Bin.,Yin, Ju-Yi.,...&Liu, Jing-fu.(2008).Development of an automated on-line pre-column derivatization procedure for sensitive determination of histamine in food with high-performance liquid chromatography-fluorescence detection.JOURNAL OF CHROMATOGRAPHY A,1209(1),70-75.
MLA Peng, Jin-feng,et al."Development of an automated on-line pre-column derivatization procedure for sensitive determination of histamine in food with high-performance liquid chromatography-fluorescence detection".JOURNAL OF CHROMATOGRAPHY A 1209.1(2008):70-75.
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