RCEES OpenIR  > 环境水质学国家重点实验室
用于分类检测水体及沉积物中四环素抗性基因tetO的引物序列及对应检测方法
强志民; 王健; 贲伟伟
2015-09-30
Rights Holder中国科学院生态环境研究中心
Date Available2015-12-09
Country中国
Abstract一种用于分类检测水体及沉积物中四环素抗性基因tetO的引物序列,包括tetO-F、tetO-R和tetO-F-GC;以及一种采用如上引物序列进行水体及沉积物中四环素抗性基因tetO的分类检测方法,通过所提供引物序列进行PCR扩增及变性梯度凝胶电泳技术,来达到分类检测tetO序列的目的。本发明通过设计引物,将PCR-DGGE技术应用于tetO序列的分类鉴定,较传统方法省去了DNA的分离纯化、载体连接及转化、阳性克隆的筛选、序列大量测序等耗工耗时耗费高的步骤,同时能得到样本中较全面的tetO序列信息,使整个操作变得简单、快速、节省成本。
Patent NumberCN105132576A
Department环境水质学国家重点实验室
Document Type专利
Identifierhttp://ir.rcees.ac.cn/handle/311016/33455
Collection环境水质学国家重点实验室
Recommended Citation
GB/T 7714
强志民,王健,贲伟伟. 用于分类检测水体及沉积物中四环素抗性基因tetO的引物序列及对应检测方法. CN105132576A[P]. 2015-09-30.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[强志民]'s Articles
[王健]'s Articles
[贲伟伟]'s Articles
Baidu academic
Similar articles in Baidu academic
[强志民]'s Articles
[王健]'s Articles
[贲伟伟]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[强志民]'s Articles
[王健]'s Articles
[贲伟伟]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.