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题名: 全氟辛基碘烷的雌激素效应及其在日本青鳉组织分布研究
作者: 王奕晨
学位类别: 硕士
答辩日期: 2011
授予单位: 中国科学院研究生院
授予地点: 北京
导师: 周群芳 ; 江桂斌
关键词: 全氟辛基碘烷 ; Perfluorooctyl iodide (PFOI) ; 卵黄蛋白原 ; Vitellogenin ; 雌激素效应 ; Estrogenic effects ; 组织分布 ; Tissue distribution
其他题名: Study on the estrogenic effects of Perfluorooctyl iodide (PFOI) and its bioaccumulation in Medaka
中文摘要:       全氟碘烷是一类氢原子被氟原子完全取代的单碘类化合物,是生产含氟类化合物的关键中间体。其中含8个碳原子的全氟辛基碘烷(Perfluorooctyl iodide,PFOI)应用最广,最具商业价值。 用PFOI分别暴露雄性日本青鳉12 h、72 h,检测肝脏中雌激素受体基因(ER α,ER β mRNA)、卵黄蛋白原基因(VTG I, VTG II mRNA)表达的变化。通过荧光实时定量PCR技术,发现12 h 暴露后,PFOI可以引起ER α、VTG I、 VTG II mRNA的显著上调,但ER β mRNA变化不明显;72 h暴露后,ER α、ER β mRNA、VTG I和VTG II mRNA显著上调,其中ER β mRNA的变化没有ER α mRNA上调显著,该结果说明PFOI可能具有雌激素活性。 用PFOI分别暴露雄性日本青鳉3 d、7 d和14 d,应用ELISA技术检测肝脏中卵黄蛋白原的生成。结果表明,PFOI可以诱导雄性日本青鳉卵黄蛋白原的生成,且随着化合物浓度增加、暴露时间延长,其诱导卵黄蛋白原生成量逐渐增加,呈现浓度效应以及时间效应关系。另外PFOI还能引起雄性日本青鳉性腺指数(GSI)的减少以及肝脏指数( HSI )的增加。 利用顶空固相微萃取-气相色谱-质谱联用检测法,对暴露14 d的日本青鳉组织样品进行含量测定。结果表明,日本青鳉肝脏、肠、性腺中均检测出PFOI,组织浓度从高至低依次为肠>肝脏>性腺。其中5 mg/L暴露组日本青鳉肠中PFOI浓度为(2428.38±608.45 ng/g),0.5 mg/L暴露组日本青鳉肠中PFOI浓度为(1156.37±616.09 ng/g)。 通过本文研究表明,全氟碘烷具有潜在的内分泌干扰效应,其生产应用及由此导致的环境污染应引起人们的高度重视,针对这类化合物开展广泛的环境风险评价势在必行。
英文摘要:       FIAs, as a kind of chemicals containing the fully fluorinated alkyl chain with an iodine atom at one end, are important intermediates in the industrial synthesis of perfluorinated compounds (PFCs). Perfluorooctyl iodide (PFOI) has high commercial value and its derivatives are widely used worldwide. The potential estrogenic activities of PFOI evaluated by monitoring changes in the expression of estrogen related genes ERa, ERb, VTG I and VTG II in the livers from medaka exposed to PFOI for 12h and 72h, using RT-PCR techniques. The present study showed the mRNA levels of ERa, VTG I and VTG II were up-regulated after exposure for 12 h, while no change was observed in ERb. But after exposure to PFOI for 72 h, the ERb is then upregulated, but the upregulation of ERb is much lower than that of ERa. The difference suggests that PFOI induces both VTG I and VTG II mRNAs in a comprehensive way. To assess the estrogenic effects of PFOI on medaka for different exposure time, the induction of hepatic VTG protein was investigated in the fish exposed to series of concentrations of PFOI for 3, 7 and 14 days, respectively. VTG protein levels increased in both dose- and time-dependent manners due to PFOI exposure. The increase of hepatic-somatic index (HSI) in a dose dependent manner and the decrease of gonadal-somatic index (GSI) were also observed in this study. Using HS-GC-MS, we quantified the concentrations of PFOI in various tissues of medaka exposed to PFOI for 14 days. Results indicated that PFOI concentrations in main target organs were in the order of intestine>liver>gonad. The highest concentration of PFOI detected in intestine exposed to 5 mg/L was 2428.38±608.45 ng/g. In 0.5mg/L group, the highest concentration of PFOI detected in intestine was 1156.37±616.09 ng/g. Based on this research, PFOI has the potential endocrine disrupting effects. We should pay much attention to this application and the resulting environmental pollution. It is necessary to carry out the risk assessment of ecotoxicological effects for this kind of compound.
内容类型: 学位论文
URI标识: http://ir.rcees.ac.cn/handle/311016/35083
Appears in Collections:环境化学与生态毒理学国家重点实验室_学位论文

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Recommended Citation:
王奕晨. 全氟辛基碘烷的雌激素效应及其在日本青鳉组织分布研究[D]. 北京. 中国科学院研究生院. 2011.
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