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题名: 氯系阻燃剂得克隆的母胎/母婴暴露研究
作者: 贲玉婕
学位类别: 硕士
答辩日期: 2012
授予单位: 中国科学院研究生院
授予地点: 北京
导师: 李兴红
关键词: 得克隆及脱氯产物 ; dechlorane plus and its dechlorinated product ; 配对人体组织样本 ; paird human tissues samples ; 产前暴露 ; pre-natal exposure ; 产后暴露 ; post-natal exposure
其他题名: Fetus/infant exposure to Dechlorane Plus and its dechlorinated products
中文摘要:       得克隆(Dechlorane Plus,DPs)具有难降解、高脂溶、生物累积和可长距离迁移的特点,已成为近年来环境研究的热点。早期的研究显示温岭地区存在较为严重的DPs污染,因此我们选择该地区作为本课题的研究区域。通过采集温岭当地捐赠者的母亲静脉血-母乳-胎盘组织-脐血的配对人体样本并收集捐赠者/新生儿相关人口信息,对DPs及其脱氯产物在母体/胎儿中的身体负荷、组织分配规律与甲状腺激素水平及其人口信息学之间的关系进行初步探索。 本研究主要开展的工作和结论如下: (1)建立了血清,母乳及胎盘组织中DPs及其两种脱氯产物(Cl10-DP和Cl11-DP)的前处理方法和GC/MS-NCI检测方法。质量控制显示,syn-DP、anti-DP及其两种脱氯产物(Cl10-DP和Cl11-DP)的添加回收率在80-110%范围内变动,相对标准偏差<15%;实际样本中13C10-syn-DP和13C10-anti-DP同位素回收率在50-110%之间变动,说明利用建立的方法的检测人体组织样本中目标分析物是具有准确性和可靠性的。 (2)根据捐赠者在温岭地区的居住时间长短,把捐赠者分为R20(生活于温岭当地>20年)和R3(生活于温岭当地<3年)两组。在R20组中,母亲静脉血、母乳、胎盘组织和脐血中的∑DPs的平均浓度分别为67.40, 37.7, 17.76和9.63 ng g-1 lw;在R3组中,∑DPs的平均浓度依次为4.26,2.34,2.46和1.90 ng g-1 lw。两组数据的几何平均值均呈现出母亲静脉血>母乳>胎盘组织>脐带血的特征;四种类型人体样本中的DPs浓度具有高度相关性(p<0.001); (3)对DPs在四种类型人体样本中的浓度分配进行两两线性拟合,R2在0.43-0.74之间; (4)R20组母亲静脉血、母乳、胎盘组织和脐血中的平均fanit分别为0.70、0.71、0.73和0.67;R3组的平均fanit依次为0.75、0.76、0.76和0.68。胎盘组织和母亲静脉血中fanti值显著高于脐带血中的fanti值, 表明DPs在从母亲静脉血转移进入脐带血的过程中,胎盘组织可能对anti-DP产生了屏障作用;DPs在乳腺组织中没有发生明显的异构体选择性富集。 (5)将产妇的人口信息包括产妇年龄、产次、职业、产前体重指数BMI、受教育水平、月经初潮年龄、月经周期与母亲静脉血中DPs浓度水平进行相关性分析。R20组的人口信息与母亲静脉血中DPs浓度水平没有发现相关性;在R3组中,母亲年龄与母亲静脉血中DPs水平显著负相关。 (6)R20组和R3组的THs之间进行独立样本T检验,发现除R20组的TSH显著低于R3组外(p=0.045),其余各项均无显著性差异。母体血清中的DPs浓度水平和五种甲状腺激素水平之间没有发现相关性(p>0.05); (7)婴儿的一般信息分组后与脐血中DPs浓度进行相关性分析,无明显相关性发现。 (8)得克隆脱一个氯的产物Cl11-DP在本研究所有母亲静脉血、母乳、胎盘组织和脐带血样本中的检出频率分别为76%、58%、67%和35%;得克隆脱两个氯的产物Cl10-DP则在所有的样本中均没有检出。 综上所述,本研究说明DPs能在母亲静脉血、母乳、胎盘组织和脐血中累积和转移;DPs及脱氯产物不仅能在母体中累积,而且可通过脐带血和母乳进一步对子代进行产前/产后暴露;DPs在四种类型样本中呈现出母亲静脉血>母乳>胎盘组织>脐带血的组织分布特征;成份特征显示胎盘组织在DPs从母亲静脉血转移到脐带血中的过程中产生了一定的anti-DP屏障作用;相比于母亲静脉血而言,DPs在母乳中没有明显的对映体选择性富集发生;母亲静脉血中DPs浓度显示与母体月经初潮年龄、月经循环周期、甲状腺激素水平等指标并不相关;脐带血中DPs浓度与胎儿发育状况指标并不相关。
英文摘要:       Due to the persistence, highly lipid solubility, bioaccumulation and long-range atmospheric transport, dechlorane plus (DPs) has been the field of focus in recent years. Wenling city, located in Taizhou, Zhejiang, is one of the famous cities involved in e-waste recycling. Our early study on the environmental safety and human health showed that there was relatively serious DPs pollution in Wenling’s environment. In Wenling region, We collected fifty paired samples including maternal serum, breast milk, placenta and cord blood serum from the same mother, who were living or had recently lived adjacent to the e-waste dismantling sites, but did not participate in e-waste dismantling operations directly, together with the donors/newborn population related information. We aimed to explore the exposure level of DPs and its dechlorinated metabolites in mother and her infant, the partitioning relationship of target compounds between paired samples, as well as investigate possible demographic influence factors on the level of contaminants and assess the effect of DPs on the maternal thyroid hormone function and body weight/gender of infant. Main contents and conclusions of our study are given in the following: Firstly, a method for the determination of DPs and the two dechlorinated metabolites (Cl10-DP and Cl11-DP)in serum, breast milk and placenta was developed by GC/MS-NCI. The spiked recoveries of syn-DP, anti-DP, Cl10-DP and Cl11-DP varied from 80-110% with the relative standard deviation of <15%. In the matrix, the recoveries of 13C10-syn-DP和13C10-anti-DP were in the range of 50-110%. The data of quality control indicated the accuracy and precision for the dtermination of target compounds in serum, breast milk and placenta. Secondly, DPs was detected in all samples. The mean of the sum of two isomers in maternal serum, breast milk, placenta and cord blood serum from the resident who had lived in the local environment over 20 years (resident >20yr group) were 67.40, 37.7, 17.76 and 9.63 ng g-1 lipid, respectively. Those from the resident who had lived in the local environment no more than 3 years (resident <3yr group) were 4.26, 2.34, 2.46 and 1.90 ng g-1 lipid, respectively. In the two groups, geomean of the concentrations of DPs showed a pattern that the level of ∑DPs in maternal serum was the highest, followed by breast milk, placenta and cord blood serum. The associated relationships was significant (p<0.001) between the compounds in different tissues from paired samples. Thirdly, for the prediction of DPs level among different tissues, the liner equation was fitted using the simple least-square method. The square of correlation coefficients was from 0.43 to 0.74. Fourthly, the mean of fanti values in maternal serum, breast milk, placenta and cord blood serum from the R20 group were 0.70, 0.71, 0.73 and 0.67, while those from the R3 group were 0.75, 0.76, 0.76 and 0.68. Compared with the value in maternal serum, breast milk and placenta, fanti of cord blood serum was significantly lower, indicating a preferential bioaccumulation of syn-DP in cord blood. Furthermore, the higher fanti value in placenta also reveal the placenta barrier in the transport process of anti-DP from maternal blood to cord blood. There were no significant difference of fanti between maternal serum and breast milk, implying the unapparent isomer selective bioaccumulation of DPs in mammary tissue. Fifthly, Nonparametric Test and Spearman Correlation Analysis were used to explore the relationship between DPs in maternal serum and demographic information( including age, occupation, parity, IBM, educational level and menstrual cycle). No significant difference was observed in R20 group, but DPs level in R3 group was observed to be correlated with maternal age, which could not be explained in our study. Sixthly, it was found that TSH in R20 group was significantly lower than that of R3 group, and the rest were no significantly different in two groups. No correlation relationship was found in the DPs level between maternal serum and THs (p>0.05). Seventhly,, there were no significant differences observed between DPs level in cord serum and fetal development index in both group. Lastly, the detection frequency of Cl11-DP in maternal serum, breast milk, placenta and cord blood serum were 76%、58%、67% and 35%, respectively. However, Cl10-DP was not detected in all samples. In conclusion, our study showed that there was relatively serious DPs contamination in the environment in Wenling. DPs could be accumulated and be transferred among human tissues. On a lipid adjusted basis, the level of DPs was in the order of maternal serum>breast milk >placenta>cord blood serum. The detection of target pollutants in breast milk and cord serum, indicating that DPs and its dechlorinated product could have a prenatal/postnatal exposure to fetus/infant. The composition of DPs in different tissues revealed that DPs and Cl11-DP have the transplacenta transfer, and syn-DP had a preferential bioaccumulation in cord blood. Compared with the value of maternal serum, there is unapprently isomeric selected bioaccumulation in mammary gland. Additionally, there were no significant correlation between DPs level in maternal serum and maternal age, occupation, parity, IBM, educational level and menstrual cycle. And, there were no significant correlation between DPs level in cord serum and fetal development index.
内容类型: 学位论文
URI标识: http://ir.rcees.ac.cn/handle/311016/35125
Appears in Collections:环境化学与生态毒理学国家重点实验室_学位论文

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Recommended Citation:
贲玉婕. 氯系阻燃剂得克隆的母胎/母婴暴露研究[D]. 北京. 中国科学院研究生院. 2012.
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