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题名: Enrichment of functional microbes and genes during pyrene degradation in two different soils
作者: Chen, Songcan; Peng, Jingjing; Duan, Guilan
刊名: JOURNAL OF SOILS AND SEDIMENTS
出版日期: 2016-02
卷号: 16, 期号:2, 页码:417-426
关键词: Abundance and diversity ; Biodegradation ; Pyrene ; Soil
DOI: 10.1007/s11368-015-1204-5
部门归属: 城市与区域生态国家重点实验室
英文摘要: Stimulating microbial degradation is a promising strategy for the remediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs). To better understand the functional microbial populations and processes involved in pyrene biodegradation in situ, the dynamics of pyrene degradation and functional microbial abundance were monitored during pyrene incubation in soils. We hope our findings will provide new insights into in situ pyrene biodegradation in soils and help to identify functional microbes from soils. Pyrene (60 mg kg(-1)) was incubated with two different soils, one is lower PAH-containing agricultural soil (LS), and the other is higher PAH-containing industrial soil (HS). During incubation, triplicate samples were collected on days 0, 3, 7, 14, and 35. Pyrene in soil samples was analyzed using an Agilent gas chromatograph (7890A) equipped with a mass-selective detector (model 5897). DNA in soils was extracted with a FastDNA Spin kit for soil (Bio101, USA). The abundance of functional microbes and genes was monitored by a Taqman or SYBR Green based real-time PCR quantification using an iCycler iQ5 themocycler (Bio-Rad, USA). The diversity of PAH-RHD alpha GP genes was evaluated by constructing clone libraries and sequencing. In both soils, more than 80 % of the added pyrene was degraded within 35 days. After 35-day incubation, there was a significant enrichment of Gram-positive bacteria harboring PAH-ring hydroxylation dioxygenase (PAH-RHD alpha GP) genes, and the abundance of Mycobacterium increased significantly. In PAH-RHD alpha GP clone libraries from two soils, Mycobacterium was detected, while most sequences were closely related to uncultured Gram-positive bacteria. In addition, two pyrene catabolic pathways might be involved in pyrene degradation, as pyrene dioxygenase genes, nidA and nidA3, were dramatically enriched during incubation. Moreover, the abundance and diversity of potential degraders in two soils showed significantly difference in responding to pyrene stress. This result indicates that soil condition can significantly affect functional microbial populations and biological process for pyrene biodegradation. These results revealed that Mycobacterium as well as uncultured Gram-positive PAH-RHD alpha genotypes may be the important group of pyrene degraders in soils, and two pyrene catabolic pathways, targeted by nidA and nidA3, might potentially contribute to in situ biodegradation of pyrene. This study characterized the response pattern of potential pyrene degraders to pyrene stress in two different soils, which would increase our understanding of the indigenous processes of pyrene biodegradation in soil environment.
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/35580
Appears in Collections:城市与区域生态国家重点实验室_期刊论文

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