中国科学院生态环境研究中心机构知识库
Advanced  
RCEES OpenIR  > 大气污染控制中心  > 期刊论文
题名: Deciphering the evolution of the functional genes and microbial community of the combined partial nitritation-anammox process with nitrate build-up and its in situ restoration
作者: Wang, Yuanyue; Wang HongYan(王红艳); Zhang, Junya; Yao, Li; Wei, Yuansong
刊名: RSC Advances
出版日期: 2016
卷号: 6, 期号:113, 页码:111702-111712
DOI: 10.1039/c6ra23865c
部门归属: 大气污染控制中心
英文摘要: The operation of a combined partial nitritation-anammox (CPNA) process was hampered by the overgrowth of nitrite-oxidizing bacteria (NOB), which was then successfully in situ restored by the combination of hydroxylamine (NH2OH) dosing and sludge retention time (SRT) control. To elucidate the microbial evolution on gene level and the microbial mechanism responding to the NO3- build-up and in situ restoration in the CPNA process, the qPCR and high-throughput sequencing were used to investigate the evolution of the N-transformation genes and microbial community in this paper. Results showed that the concentrations of amoA and hao were both decreased as well as the hzo along with the NO3- build-up, however, the nitrite-oxidation encoding gene nxrA was increased. Pearson correlation analysis demonstrated that the gene nxrA was significantly correlated with the NO3-. After restoration, the abundances of amoA, hao and hzo were increased to 26.58%, 43.56% and 26.17%, respectively, while the growth of nxrA was effectively limited. The obtained high abundance of narG gene (37.85%) as well as the significant correlation of hzo-narG and hzo-nirS illustrated the in situ restoring mechanism, i.e., the intensive denitrification of the accumulated NO3- to NO followed by the stimulation of the anammox by NO. Additionally, MiSeq sequencing showed a new microbial community was rebuilt after restoration and the Haliscomenobacter and Nitrosomonas were the dominant genera. The abundance of anammox genus Candidatus Kuenenia was also increased to 8.0%. The significant correlation between nirK and Ignavibacterium indicated that the filaments played an important role in the denitrification of the accumulated NO3-.
收录类别: SCI
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/35739
Appears in Collections:大气污染控制中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Deciphering the evolution of the functional genes and microbial community of the combined partial nitritation-anammox process with nitrate build-up and its in situ restoration.pdf(1186KB)期刊论文作者接受稿开放获取View Download
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Yuanyue]'s Articles
[Wang, Hongyan]'s Articles
[Zhang, Junya]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Yuanyue]‘s Articles
[Wang, Hongyan]‘s Articles
[Zhang, Junya]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Deciphering the evolution of the functional genes and microbial community of the combined partial nitritation-anammox process with nitrate build-up and its in situ restoration.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院生态环境研究中心 - Feedback
Powered by CSpace