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题名: Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii
作者: Wang, Songshan; Lv, Jitao; Ma, Jingyuan; Zhang, Shuzhen
刊名: NANOTOXICOLOGY
出版日期: 2016
卷号: 10, 期号:8, 页码:1129-1135
关键词: Chlamydomonas reinhardtii ; HAADF-STEM ; NanoSIMS ; silver nanoparticles ; XAS
DOI: 10.1080/17435390.2016.1179809
部门归属: 环境化学与生态毒理学国家重点实验室
英文摘要: It is necessary to elucidate cellular internalization and intracellular biotransformation in order to accurately assess the toxicity and fate of nanoparticles after interaction with organisms. Therefore, this work employed a combination of high resolution imaging and in situ detection spectroscopic techniques to systematically investigate the intracellular localization, morphology and chemical speciation of silver in the cells of Chlamydomonas reinhardtii, a unicellular freshwater green alga, after exposure to AgNPs coated with polyvinylpyrrolidone at a concentration of 2.0mg/L. High resolution secondary ion mass spectrometry and high-angle annular dark field scanning transmission electron microscopy together with energy dispersive spectroscopy and selected area electron diffraction collectively confirmed that after 48h of exposure, AgNPs entered the periplasmic space after cellular internalization into the algal cells. Silver was also found to coexist with sulfur inside the cytoplasm in both crystalline and amorphous forms, which were further identified as -Ag2S and silver thiolates with synchrotron X-ray absorption spectroscopy. In combination, these analyses demonstrated that silver inside algae could be attributed to the uptake and sequestration of Ag(+)ion released from AgNPs, which was further sequestrated into cellular compartments. This study provides solid evidence for particle internalization and biotransformation of AgNPs after interaction with algae.
收录类别: SCI
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/35815
Appears in Collections:环境化学与生态毒理学国家重点实验室_期刊论文

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