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题名: Detection of 1,N-2-propano-2 '-deoxyguanosine in human urine by stable isotope dilution UHPLC-MS/MS analysis
作者: Zhang, Ning; Song, Yuanyuan; Zhang, Weibing; Wang, Hailin
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期: 2016-06-15
卷号: 1023, 期号:0, 页码:68-71
关键词: 1,N-2-propano-2 '-deoxyguanosine adducts ; Human urine ; SPE ; UHPLC-MS/MS
DOI: 10.1016/j.jchromb.2016.04.029
部门归属: 环境化学与生态毒理学国家重点实验室
英文摘要: A sensitive and accurate stable isotope dilution UHPLC-MS/MS method was developed and validated for the detection and quantification of ProdG adducts in human urine, a surrogate for the ProdG adducts in genomic DNA of human. A specific solid phase extraction (SPE) approach was established for selective enrichment of urinary ProdG adducts and elimination of urinary matrix facilitating the coupled MS/MS detection. The recovery of the method is estimated about 84.8-107.2%, and the precision are about 0.8-3.6% for intraday and 2.8-10.0% for interday. Due to that the matrix effect is efficiently eliminated by SPE pretreatment, the limits of detection (LODs, S/N = 3) and quantification (LOQs, S/N = 10) are decreased to 100 and 300 amol for urinary ProdG adducts, respectively. By coupling the developed SPE pretreatment with the UHPLC-MS/MS analysis, ProdG adducts were accurately quantified in healthy human urine. (C) 2016 Elsevier B.V. All rights reserved.
收录类别: SCI
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/35832
Appears in Collections:环境化学与生态毒理学国家重点实验室_期刊论文

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Recommended Citation:
Zhang, Ning,Song, Yuanyuan,Zhang, Weibing,et al. Detection of 1,N-2-propano-2 '-deoxyguanosine in human urine by stable isotope dilution UHPLC-MS/MS analysis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1023(0):68-71.
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文件名: Detection of 1,N-2-propano-2 '-deoxyguanosine in human urine by stable isotope dilution UHPLC-MSMS analysis.pdf
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