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题名: Detection of of 1,N-2-propano-2 '-deoxyguanosine adducts in genomic DNA by ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry in combination with stable isotope dilution
作者: Zhang, Ning; Song, Yuanyuan; Wu, Danni; Xu, Tian; Lu, Meiling; Zhang, Weibing; Wang, Hailin
刊名: JOURNAL OF CHROMATOGRAPHY A
出版日期: 2016-06-10
卷号: 1450, 期号:0, 页码:38-44
关键词: Crotonaldehyde (Cro) ; 1,N-2-propano-2'-deoxyguanosine adducts (ProdG adducts) ; Ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS)
DOI: 10.1016/j.chroma.2016.04.067
部门归属: 环境化学与生态毒理学国家重点实验室
英文摘要: Crotonaldehyde (Cro) is one of widespread and genotoxic alpha,beta-unsaturated aldehydes and can react with the exocyclic amino group of 2'-deoxyguanosine (dG) in genomic DNA to form 1,N-2-propano-2'-deoxyguanosine (ProdG) adducts. In this study, two diastereomers of high purity were prepared, including non-isotope and stable isotope labeled ProdG adducts, and exploited stable isotope dilution-based calibration method. By taking advantage of synthesized ProdG standards, we developed a sensitive ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) method for accurate quantification of two diastereomers of ProdG adducts. In addition to optimization of the UHPLC separation, ammonium bicarbonate (NH4HCO3) was used as additive in the mobile phase for enhancing the ionization efficiency to ProdG adducts and facilitating MS detection. The limits of detection (LODs, S/N=3) and the limits of quantification (LOQs, S/N = 10) are estimated about 50 amol and 150 amol, respectively. By the use of the developed method, both diastereomers of ProdG adducts can be detected in untreated human MRC5 cells with a frequency of 2.4-3.5 adducts per 10(8) nucleotides. Crotonaldehyde treatment dramatically increases the levels of ProdG adducts in human MRC5 in a concentration-dependent manner. (C) 2016 Elsevier B.V. All rights reserved.
收录类别: SCI
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/35833
Appears in Collections:环境化学与生态毒理学国家重点实验室_期刊论文

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Recommended Citation:
Zhang, Ning,Song, Yuanyuan,Wu, Danni,et al. Detection of of 1,N-2-propano-2 '-deoxyguanosine adducts in genomic DNA by ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry in combination with stable isotope dilution[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1450(0):38-44.
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文件名: Detection of 1,N-2-propano-2 '-deoxyguanosine adducts in genomic DNA by ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry .pdf
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