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题名: Fluorescence anisotropy assay for D-vasopressin with a tetramethylrhodamine-labeled aptamer
作者: Liu, Yingxiong; Zhao, Qiang
刊名: ANALYTICAL METHODS
出版日期: 2016-01
卷号: 8, 期号:11, 页码:2383-2390
DOI: 10.1039/c6ay00315j
部门归属: 环境化学与生态毒理学国家重点实验室
英文摘要: Here we report a fluorescence anisotropy (FA) assay for D-vasopressin (D-Vas), an important active peptide, by using a tetramethylrhodamine (TMR) labeled 55-nt aptamer that contains 19 guanine (G) bases. This assay is based on a target-binding induced change of the intramolecular interaction between TMR and G base and the subsequent FA alteration of labeled TMR. We conjugated single TMR on different labeling positions of thymine (T) bases or terminals of the aptamer, obtaining 14 TMR-labeled aptamers. We screened the FA response of each TMR-labeled aptamer upon D-Vas addition. An aptamer probe with TMR labeled on the 34th base T in sequence exhibited sensitive and significant FA-decreasing responses upon the binding of D-Vas. Under optimized assay conditions this favorable TMR-labeled aptamer allowed direct FA detection of D-Vas ranging from 0.2 mu M to 200 mu M with good selectivity. This FA method also enabled the detection of D-Vas spiked in diluted serum and urine samples.
收录类别: SCI
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/35857
Appears in Collections:环境化学与生态毒理学国家重点实验室_期刊论文

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