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题名: Molecular cloning, characterization, and the response of Cu/Zn superoxide dismutase and catalase to PBDE-47 and-209 from the freshwater bivalve Anodonta woodiana
作者: Xia, Xichao; Huang, Chuanfeng; Zhang, Dongxian; Zhang, Yi; Xue, Shipeng; Wang, Xiying; Zhang, Qingyuan; Guo, Lianghong
刊名: FISH & SHELLFISH IMMUNOLOGY
出版日期: 2016-04
卷号: 51, 期号:0, 页码:200-210
关键词: Anodonta woodiana ; AwSOD ; AwCAT ; PBDE-47 ; PBDE-209
DOI: 10.1016/j.fsi.2016.02.025
部门归属: 环境化学与生态毒理学国家重点实验室
英文摘要: Polybrominated diphenyl ethers-47 (PBDE-47) and -209 are significant components of total PBDEs in water and can catalyze the production of reactive oxygen species (ROS) in the organisms. Anti-oxidant enzymes play an important role in scavenging the high level of ROS. In the current study, two full-length cDNAs of Cu/Zn superoxide dismutase (CuZnSODs) and catalase (CAT) were isolated from freshwater bivalve Anodonta woodiana by rapid amplification of cDNA ends approach and respectively named as AwSOD and AwCAT. The nucleotide sequence of AwSOD cDNA had an open reading frame (ORF) of 465 bp encoding a polypeptide of 155 amino acids in which signature 1 GKHGFHVHEFGDNT and signature 2 GNAGARSACGVI of SODs were observed. Deduced amino acid sequence of AwSOD showed a significant similarity with that of CuZnSODs. AwCAT had an ORF 1536 bp encoding a polypeptide of 512 amino acids which contains a conserved catalytic site motif, and a proximal heme-ligand signature motif of CATs. The time-course expressions of AwSOD and AwCAT in hepatopancreas were measured by quantitative real-time PCR. Expressions of AwSOD and AwCAT showed a significant up-regulation in groups at a low concentration treatment of PBDE-47, a biphasic pattern in groups with a high concentration treatment. Administration of PBDE-209 could result in an up-regulation of AwSOD and AwCAT expressions with time- and dose-dependent matter. These results indicate that up-regulations of AwSOD and AwCAT expression of hepatopancreas of freshwater bivalve A. woodiana contribute to eliminate oxidative stress derived from PBDE-47 and-209 treated. (C) 2016 Elsevier Ltd. All rights reserved.
收录类别: SCI
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/35899
Appears in Collections:环境化学与生态毒理学国家重点实验室_期刊论文

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Recommended Citation:
Xia, Xichao,Huang, Chuanfeng,Zhang, Dongxian,et al. Molecular cloning, characterization, and the response of Cu/Zn superoxide dismutase and catalase to PBDE-47 and-209 from the freshwater bivalve Anodonta woodiana[J]. FISH & SHELLFISH IMMUNOLOGY,2016,51(0):200-210.
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