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题名: Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1
作者: Yin, Kun; Lv, Min; Wang, Qiaoning; Wu, Yixuan; Liao, Chunyang; Zhang, Weiwei; Chen, Lingxin
刊名: WATER RESEARCH
出版日期: 2016-10-15
卷号: 103, 期号:0, 页码:383-390
关键词: Carboxylesterase E2 ; Surface display ; Mercury ; Bioremediation ; Biodetection
DOI: 10.1016/j.watres.2016.07.053
部门归属: 环境化学与生态毒理学国家重点实验室
英文摘要: Mercury is a toxic heavy metal and presents significant threats to organisms and natural ecosystems. Recently, the mercury remediation as well as its detection by environmental-friendly biotechnology has received increasing attention. In this study, carboxylesterase E2 from mercury-resistant strain Pseudomonas aeruginosa PA1 has been successfully displayed on the outer membrane of Escherichia coli Top10 bacteria to simultaneously adsorb and detect mercury ion (Hg2+). The transmission electron microscopy analysis shows that Hg2+ can be absorbed by carboxylesterase E2 and accumulated on the outer membrane of surface-displayed E. coli bacteria. The adsorption of Hg2+ followed a physicochemical, equilibrated and saturatable mechanism, which well fits the traditional Langmuir adsorption model. The surface-displayed system can be regenerated through regulating pH values. As its activity can be inhibited by Hg2+, carboxylesterase E2 has been used to detect the concentration of Hg2+ in water samples. The developed surface display system will be of great potential in the simultaneous bioremediation and biodetection of environmental mercury pollution. (C) 2016 Elsevier Ltd. All rights reserved.
收录类别: SCI
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/35945
Appears in Collections:环境化学与生态毒理学国家重点实验室_期刊论文

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