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题名: T-screen and yeast assay for the detection of the thyroid-disrupting activities of cadmium, mercury, and zinc
作者: Li, Jian; Liu, Yun; Kong, Dongdong; Ren, Shujuan; Li, Na
刊名: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
出版日期: 2016-05
卷号: 23, 期号:10, 页码:9843-9851
关键词: In vitro bioassay ; Toxicmetals ; Thyroid receptor ; Thyroid-disrupting activity
DOI: 10.1007/s11356-016-6095-5
部门归属: 环境水质学国家重点实验室
英文摘要: In the present study, a two-hybrid yeast bioassay and a T-screen were used to screen for the thyroid receptor (TR)-disrupting activity of select metallic compounds (CdCl2, ZnCl2, HgCl2, CuSO4, MnSO4, and MgSO4). The results reveal that none of the tested metallic compounds showed TR-agonistic activity, whereas ZnCl2, HgCl2, and CdCl2 demonstrated TR antagonism. For the yeast assay, the dose-response relationship of these metallic compounds was established, and the concentrations producing 20 % of the maximum effect of ZnCl2, HgCl2, and CdCl2 were 9.1 x 10(-5), 3.2 x 10(-6), and 1.2 x 10(-6) mol/L, respectively. The T-screen also supported the finding that ZnCl2, HgCl2, and CdCl2 decreased the cell proliferation at concentrations ranging from 10(-6) to 10(-4) mol/L. Furthermore, the thyroid-disrupting activity of metallic compounds in environmental water samples collected from the Guanting Reservoir, Beijing, China was evaluated. Solid-phase extraction was used to separate the organic extracts, and a modified two-hybrid yeast bioassay revealed that the metallic compounds in the water samples could affect thyroid hormone-induced signaling by decreasing the binding of the thyroid hormone. The addition of ethylenediaminetetraacetic acid (30 mg/L) could eliminate the effects. Thus, the cause(s) of the thyroid toxicity in the water samples appeared to be partly related to the metallic compounds.
收录类别: SCI
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/36085
Appears in Collections:环境水质学国家重点实验室_期刊论文

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