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题名: Optimization of key factors affecting biohydrogen production from microcrystalline cellulose by the co-culture of Clostridium acetobutylicum X-9 + Ethanoigenens harbinense B-2
作者: Bao, Hongxu; Chen, Chunxiao; Jiang, Lei; Liu, Yichen; Shen, Manli; Liu, Wenzong; Wang, Aijie
刊名: RSC ADVANCES
出版日期: 2016-01
卷号: 6, 期号:5, 页码:3421-3427
DOI: 10.1039/c5ra14192c
部门归属: 中国科学院环境生物技术重点实验室
英文摘要: The key factors (initial pH value, substrate concentration, incubation time, C/N and L-cysteine concentration) that affect biohydrogen production from microcrystalline cellulose in batch fermentation by the co-culture of isolated strains (Clostridium acetobutylicum X-9 + Ethanoigenens harbinense B-2) were optimized using an orthogonal experiment. The isolated strain, Clostridium acetobutylicum X-9, had high hydrogen yield from microcrystalline cellulose (MCC), and Ethanoigenens harbinense B-2 could produce hydrogen efficiently from monosaccharides directly from microcrystalline cellulose. The optimal parameters were as follows: initial pH value of 6.0, 12 g L-1 substrate concentration, 40 h incubation time, 0.7 g L-1 L-cysteine concentration and a 4 : 1 ratio of C/N. Under the optimum culture conditions, a maximum hydrogen yield rate of 10.4 mmol g-MCC-1 was obtained. This yield is approximately 2.2-fold greater than that of mono-culture Clostridium acetobutylicum X-9. It suggests that the optimal conditions achieved can be applied to the production of hydrogen from microcrystalline cellulose using the co-culture of isolated strains, Clostridium acetobutylicum X-9 + Ethanoigenens harbinense B-2.
收录类别: SCI
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/36138
Appears in Collections:中国科学院环境生物技术重点实验室_期刊论文

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