中国科学院生态环境研究中心机构知识库
Advanced  
RCEES OpenIR  > 中国科学院环境生物技术重点实验室  > 期刊论文
题名: Reference gene selection for quantitative gene expression studies during biological invasions: A test on multiple genes and tissues in a model ascidian Ciona savignyi
作者: Huang, Xuena; Gao, Yangchun; Jiang, Bei; Zhou, Zunchun; Zhan, Aibin
刊名: GENE
出版日期: 2016-01-15
卷号: 576, 期号:1, 页码:79-87
关键词: Ascidian ; Biological invasions ; Ciona savignyi ; Gene expression ; Reference genes ; qRT-PCR
DOI: 10.1016/j.gene.2015.09.066
部门归属: 中国科学院环境生物技术重点实验室
英文摘要: As invasive species have successfully colonized a wide range of dramatically different local environments, they offer a good opportunity to study interactions between species and rapidly changing environments. Gene expression represents one of the primary and crucial mechanisms for rapid adaptation to local environments. Here, we aim to select reference genes for quantitative gene expression analysis based on quantitative Real-Time PCR (qRT-PCR) for a model invasive ascidian, Ciona savignyi. We analyzed the stability of ten candidate reference genes in three tissues (siphon, pharynx and intestine) under two key environmental stresses (temperature and salinity) in the marine realm based on three programs (geNorm, NormFinder and delta Ct method). Our results demonstrated only minor difference for stability rankings among the three methods. The use of different single reference gene might influence the data interpretation, while multiple reference genes could minimize possible errors. Therefore, reference gene combinations were recommended for different tissues - the optimal reference gene combination for siphon was RPS15 and RPL17 under temperature stress, and RPL17, UBQ and TubA under salinity treatment; for pharynx, TubB, TubA and RPL17 were the most stable genes under temperature stress, while TubB, TubA and UBQ were the best under salinity stress; for intestine, UBQ, RPS15 and RPL17 were the most reliable reference genes under both treatments. Our results suggest that the necessity of selection and test of reference genes for different tissues under varying environmental stresses. The results obtained here are expected to reveal mechanisms of gene expression-mediated invasion success using C savignyi as a model species. (C) 2015 Elsevier B.V. All rights reserved.
收录类别: SCI
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/36142
Appears in Collections:中国科学院环境生物技术重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Reference gene selection for quantitative gene expression studies during biological invasions A test on multiple genes and tissues in a model ascidian Ciona savignyi.pdf(624KB)期刊论文作者接受稿开放获取View Download

Recommended Citation:
Huang, Xuena,Gao, Yangchun,Jiang, Bei,et al. Reference gene selection for quantitative gene expression studies during biological invasions: A test on multiple genes and tissues in a model ascidian Ciona savignyi[J]. GENE,2016,576(1):79-87.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Huang, Xuena]'s Articles
[Gao, Yangchun]'s Articles
[Jiang, Bei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Huang, Xuena]‘s Articles
[Gao, Yangchun]‘s Articles
[Jiang, Bei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Reference gene selection for quantitative gene expression studies during biological invasions A test on multiple genes and tissues in a model ascidian Ciona savignyi.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院生态环境研究中心 - Feedback
Powered by CSpace