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题名: Mechanisms of L-lysine extraction with sec-octylphenoxy acetic acid in sulfonated kerosene
作者: Zhao, Chunhua; Zhou, Xiaohua; Xiao, Yan; Wang, Dan; Zhou, Zhen; Yang, Zhixiong; Jiang, Xiaoqin; Wang, Jiang
刊名: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
出版日期: 2016-07
卷号: 91, 期号:7, 页码:2047-2055
关键词: L-lysine ; sec-octylphenoxy acetic acid ; extraction mechanism ; electrostatic interaction ; AFM
DOI: 10.1002/jctb.4799
部门归属: 中国科学院饮用水科学与技术重点实验室
英文摘要: BACKGOUNDCurrently, the dominant theories on extraction mechanisms of amino acids are mainly deduced from mathematical models or reaction equations, it is thus necessary to obtain direct evidence for the extraction mechanisms by experimental methods. This work aims to reveal the mechanisms of L-lysine (L-Lys) extraction with sec-octylphenoxy acetic acid (CA-12) in sulfonated kerosene by atomic force microscopy (AFM), half-saturated fluorometric experiment and competition experiment. RESULTSThe ionic bond based on the electrostatic attraction between the amino group of L-Lys and the carboxyl group of CA-12 was verified visually by high-resolution AFM. Half of the amino groups of L-Lys were labeled by o-phthalaldehyde (OPA) to form half-saturated fluorescent derivatives which were then extracted by means of CA-12 in sulfonated kerosene. The electrostatic attraction between the free amino group of L-Lys and the carboxyl group of CA-12 was illustrated by the relationships between OPA dosage and fluorescence intensities of stock solution and extracted liquid. This electrostatic interaction was also demonstrated by the competition experiment using three other amino acids including L-arginine, L-aspartic acid and L-alanine as competitive reagents. CONCLUSIONThe chemical force of L-Lys extraction with CA-12 in sulfonated kerosene was comprehensively demonstrated to be the electrostatic interaction between the amino group and the carboxyl group. (c) 2015 Society of Chemical Industry
收录类别: SCI
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/36197
Appears in Collections:中国科学院饮用水科学与技术重点实验室_期刊论文

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