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题名: Augmentation of protein-derived acetic acid production by heat-alkaline-induced changes in protein structure and conformation
作者: Wang, Xu; Li, Yanbo; Liu, Junxin; Ren, Nan-Qi; Qu, Jiuhui
刊名: WATER RESEARCH
出版日期: 2016-01
卷号: 88, 页码:595-603
关键词: Waste activated sludge ; Fermentation ; Pretreatment ; Acetic acid ; Protein structure and conformation ; Economic analysis
DOI: 10.1016/j.watres.2015.10.055
部门归属: 水污染控制实验室
英文摘要: Waste-derived acetic acid (HAc) is an attractive feedstock for microbe-mediated biofuel production. However, fermentative conversion of HAc from waste-activated sludge (WAS) has low yield because of the high concentration of proteins not readily utilizable by microorganisms without prior hydrolysis. We investigated a combined technology for HAc augmentation during sludge protein fermentation. The maximal HAc yield increased over two-fold, reaching 0.502 +/- 0.021 g/g protein (0.36 +/- 0.01 g COD/g COD, similar to 52% of the total volatile fatty acids) when synthetic sludge protein was heated at 120 degrees C for 30 min, treated at pH 12 for 24 h, and fermented at pH 9 for 72 h. Comprehensive analysis illustrated that the heat-alkaline pretreatment significantly induced protein fragmentation, simultaneously increasing the efficiency of protein biohydrolysis (from 35.5% to 85.9%) by inducing conformational changes indicative of protein unfolding. Consequently, the native alpha-helix content was decreased from 67.3% to 32.5% by conversion to an unordered shape, whose content increased from 27.5% to 45.5%; disulfide bonds were cleaved, whereas the main S-S stretching pattern was altered from gauche-gauche-gauche to gauche-gauche-trans, consequently causing increased protein susceptibility to proteolytic hydrolysis (76.3% vs. 47.0%). Economic analysis indicated that anaerobic fermentation with appropriate heat-alkaline pretreatment is a cost-effective approach for waste conversion to energy sources such as HAc. (C) 2015 Elsevier Ltd. All rights reserved.
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内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/36228
Appears in Collections:水污染控制实验室_期刊论文

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