中国科学院生态环境研究中心机构知识库
Advanced  
RCEES OpenIR  > 土壤环境科学实验室  > 期刊论文
题名: Long-term field application of sewage sludge increases the abundance of antibiotic resistance genes in soil
作者: Chen, Qinglin; An, Xinli; Li, Hu; Su, Jianqiang; Ma, Yibing; Zhu, Yong-Guan
刊名: ENVIRONMENT INTERNATIONAL
出版日期: 2016-08
卷号: 92-93, 页码:1-10
关键词: Antibiotic resistance genes ; Bacterial pathogens ; Horizontal gene transfer ; Network analysis ; Sewage sludge
DOI: 10.1016/j.envint.2016.03.026
部门归属: 土壤环境科学实验室
英文摘要: Sewage sludge and manure are common soil amendments in crop production; however, their impact on the abundance and diversity of the antibiotic resistome in soil remains elusive. In this study, by using high throughput sequencing and high-throughput quantitative PCR, the patterns of bacterial community and antibiotic resistance genes (ARGs) in a long-term field experiment were investigated to gain insights into these impacts. A total of 130 unique ARGs and 5 mobile genetic elements (MGEs) were detected and the long-term application of sewage sludge and chicken manure significantly increased the abundance and diversity of ARGs in the soil. Genes conferring resistance to beta-lactams, tetracyclines, and multiple drugs were dominant in the samples. Sewage sludge or chicken manure applications caused significant enrichment of 108 unique ARGs and MGEs with a maximum enrichment of up to 3845 folds for mexF. The enrichment of MGEs suggested that the application of sewage sludge or manure may accelerate the dissemination of ARGs in soil through horizontal gene transfer (HGT). Based on the co-occurrence pattern of ARGs subtypes revealed by network analysis, aacC, oprD and mphA-02, were proposed to be potential indicators for quantitative estimation of the co-occurring ARGs subtypes abundance by-power functions. The application of sewage sludge and manure resulted in significant increase of bacterial diversity in soil, Proteobacteria, Acidobacteria, Actinobacteria and Chloroflexi were the dominant phyla (>10% in each sample). Five bacterial phyla (Chloroflexi, Planctomycetes, Firmicutes, Gemmatimonadetes and Bacteroidetes) were found to be significantly correlated with the ARGs in soil. Mantel test and variation partitioning analysis (VPA) suggested that bacterial community shifts, rather than MGEs, is the major driver shaping the antibiotic resistome. Additionally, the co-occurrence pattern between ARGs and microbial taxa revealed by network analysis indicated that four bacterial families might be potential hosts of ARGs. These results may shed light on the mechanism underlining the effects of amendments of sewage sludge or manure on the occurrence and dissemination of ARGs in soil. (C) 2016 Elsevier Ltd. All rights reserved.
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/36259
Appears in Collections:土壤环境科学实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Long-term field application of sewage sludge increases the abundance of antibiotic resistance genes in soil.pdf(1891KB)期刊论文作者接受稿开放获取View Download
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen, Qinglin]'s Articles
[An, Xinli]'s Articles
[Li, Hu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen, Qinglin]‘s Articles
[An, Xinli]‘s Articles
[Li, Hu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Long-term field application of sewage sludge increases the abundance of antibiotic resistance genes in soil.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院生态环境研究中心 - Feedback
Powered by CSpace