中国科学院生态环境研究中心机构知识库
Advanced  
RCEES OpenIR  > 环境水质学国家重点实验室  > 期刊论文
题名: 微丝菌(Microthrix parvicella)原位荧光杂交(FISH)定量过程的条件优化
作者: 王润芳; 张红; 王琴; 王娟; 顾剑; 齐嵘; 杨敏
刊名: 环境科学
出版日期: 2016
卷号: 37, 期号:6, 页码:2266-2270
关键词: 污泥膨胀 ; 微丝菌 ; FISH技术 ; 优化 ; 定量
其他题名: Optimization for Microthrix parvicella Quantitative Processing of Fluorescence in situ Hybridization (FISH)
中文摘要: 微丝菌(Microthrix parvicella)是世界范围内诱发活性污泥膨胀现象的主要丝状菌之一,它在活性污泥中准确的原位定量解析对污泥膨胀现象及控制策略研究具有非常重要的意义. 由于微丝菌自身的特殊生理生化性质(如表面高疏水性及较厚细胞壁)易导致常规荧光原位杂交(FISH)过程中定量结果偏低. 本研究针对FISH过程中存在的探针渗透率低、 荧光信号偏弱等现象, 从活性污泥样品前处理、 杂交过程条件等方面对Microthrix parvicella的FISH定量过程进行了优化. 结果表明,在前处理使用溶菌酶(浓度为36000 U·mL~(-1)),探针浓度为4.5 ng·muL~(-1),杂交时间延长至4 h的条件下,Microthrix parvicella的FISH定量结果可从1.12%提高至96.70%,并与定量PCR(q-PCR)结果和Eikelboom & Jenkins法(镜检观察)定量结果更为趋近一致.
部门归属: 环境水质学国家重点实验室
英文摘要: Precise quantification of Microthrix parvicella, which is identified as a dominated filamentous bacterium of bulking sludge in the worldwide, is essential for bulking investigation and related control strategies. However, quantitative processing based on fluorescence in situ hybridization (FISH) is prone to interference due to the specific characteristics of Microthrix parvicella (hydrophobic surface with thick cell wall). Our study focused on pretreatment and process optimization to show that the proportion of Microthrix parvicella was increased from 1.12% to 96.70% benefited by lysozyme (36000 U·mL~(-1)), high probe concentration (4.5 ng·muL~(-1)) and longer hybridization time (4 h) employed, mapping with the results of q-PCR method and Eikelboom & Jenkins Observation.
收录类别: CSCD
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.rcees.ac.cn/handle/311016/36666
Appears in Collections:环境水质学国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
微丝菌_Microthrixpa_省略_交_FISH_定量过程的条件优化_王润芳.pdf(489KB)期刊论文作者接受稿开放获取View Download

Recommended Citation:
王润芳,张红,王琴,等. 微丝菌(Microthrix parvicella)原位荧光杂交(FISH)定量过程的条件优化[J]. 环境科学,2016,37(6):2266-2270.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[王润芳]'s Articles
[张红]'s Articles
[王琴]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[王润芳]‘s Articles
[张红]‘s Articles
[王琴]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 微丝菌_Microthrixpa_省略_交_FISH_定量过程的条件优化_王润芳.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院生态环境研究中心 - Feedback
Powered by CSpace