中国科学院生态环境研究中心机构知识库
Advanced  
RCEES OpenIR  > 环境水质学国家重点实验室  > 学位论文
题名: 羟胺和肼调控一体式部分亚硝化-厌氧氨氧化工艺的对比研究
作者: 王元月1
学位类别: 博士后
答辩日期: 2017-07
授予单位: 中国科学院大学
授予地点: 北京
导师: 单保庆 ; 魏源送
关键词: 厌氧氨氧化,短程硝化,羟胺,肼,亚硝酸盐氧化菌 ; anammox, partial nitritation, hydroxylamine, hydrazine, nitrite-oxidizing bacteria
学位专业: 生物学
中文摘要: 厌氧氨氧化是目前最有应用前景的新型生物脱氮技术。然而,该技术在实际工程应用中,常因亚硝酸盐氧化菌(NOB)的过量繁殖而导致系统内出现NO3--N累积、厌氧氨氧化活性下降等异常情况发生,最终会使脱氮效率恶化,运行趋于崩溃。 针对NOB过量繁殖问题,传统的应对措施如降低溶解氧、缩减污泥龄甚至排空污泥重新接种启动等存在恢复周期长,或者因菌种获取困难而没有普遍性等问题。近年来,有学者提出了利用羟胺(NH2OH)和肼(N2H4)投加,可以达到原位抑制NOB、提高厌氧氨氧化菌活性的双重目的,最终能够在较短时间内恢复系统的脱氮功能。然而,已有研究主要关注NH2OH和N2H4投加后的直接运行效果,还缺乏从功能基因、微生物群落结构等深层次的机理探究。因此,本研究以一体式部分亚硝化-厌氧氨氧化SBR工艺(Combined partial nitritation-anammox, CPNA)为对象,通过投加NH2OH和N2H4,对比考察两者在NOB过量繁殖下的原位恢复效果,并从微生物层面解析其作用机理。 (1)构建三组SBR硝化系统,对比考察NH2OH和N2H4投加对实现NO2--N累积的作用效果。结果表明投加NH2OH的1#SBR最快实现NO2--N累积,且累积率最高可达85.95%。NH2OH对氨氧化菌(AOB)活性的促进作用要强于N2H4,N2H4对NOB活性的抑制存在一定程度的可逆性。 (2)针对CPNA工艺中出现的NOB过量繁殖问题,投加NH2OH对NOB的抑制效果要好于N2H4。投加NH2OH的1#SBR出水中的NO3--N可以从最初的327.08 mg/L降低至93.31 mg/L,相应的脱氮速率则能够恢复至0.2 kgN/m3.d以上。而投加N2H4的2#SBR和对照组3#SBR的出水NO3--N浓度仍高达190.14 mg/L和152.49 mg/L。对于SBR单周期内的NO3--N产生与NH4+-N去除之间摩尔比(mole ratio of NO3--N production to NH4+-N reduced, MRNN),三组SBR分别从最初的69.78%、72.43%和64.67%恢复至19.91%、41.02%和32.96%。停止投加N2H4之后,2#SBR会再次出现一定程度的NO3--N累积。污泥SVI值逐渐增大,丝状菌滋生。 (3)在投加NH2OH成功实现对CPNA工艺的原位在线恢复过程中,编码氨氧化过程的amoA、hao基因以及厌氧氨氧化过程主要基因hzo的丰度能够分别提高至26.58%、43.56%和26.17%。微生物群落多样性指数呈下降趋势,且丝状菌丰度得到大幅提高。丝状菌能够发挥一定反硝化功能,将NO3--N还原为NO2--N,作为厌氧氨氧化反应底物,也就与NH2OH直接抑制NOB活性相协同,缓解系统的NO3--N累积现象,原位恢复系统的脱氮功能。
英文摘要: Anaerobic ammonium oxidation (ANAMMOX) is a novel nitrogen removal technology with great application prospect. However, during the engineering application of this new technology, the overgrowth of nitrite-oxidizing bacteria (NOB) happened frequently, which would cause the build-up of nitrate and reduce the anammox activity. Finally, the overall nitrogen removal performance could be deteriorated and the long-term stable operation collapsed. As for the problem of overgrowth of NOB, due to the relative long term restoration period or the lack of sufficient new seeding sludge, the conventional countermeasures, such as the reduction of dissolved oxygen, shortening the sludge retention time (SRT) and draining out the reactor and re-innoculation were not universal. Recently, some researchers had proposed that through dosing the hydroxylamine (NH2OH) and hydrazine (N2H4) to system, the NOB could be in-situ inhibited, the anammox activity could be stimulated, and finally the nitrogen removal could be recovered. However, nearly all the existing studies were focused on the direct operational performance, there were no researches aiming at exploring the restoration mechanisms on the level of microbial gene and community. Therefore, the purpose of this study was to investigate the in-situ restoration performance and mechanisms by dosing the NH2OH and N2H4 to a combined partial nitritation-anammox process (CPNA), which was tangled by the overgrowth of NOB. (1) Three SBR nitrifying systems were set to investigate the promotion effect of nitrite accumulation by dosing NH2OH and N2H4 parallelly. The results showed that nitrite accumulation was firstly observed in the 1#SBR with NH2OH, and the highest 85.95% of nitrite accumulation rate was obtained. NH2OH showed higher stimulation effect on the anammox bacteria than N2H4, and the inhibition of N2H4 to NOB was relatively reversible. (2) For the NOB problem in the combined partial nitritation-anammox process, the in-situ restoration performance by dosing NH2OH was better than dosing N2H4. The nitrate concentration in the effluent of 1#SBR (dosing NH2OH) decreased from the initial 327.08 mg/L to 93.31 mg/L, and more than 0.2 kgN/m3.d of TN removal rate was recovered. The concentrations of nitrate from the effluents of 2#SBR (dosing N2H4) and 3#SBR (blank control) were still as high as 190.14 mg/L and 152.49 mg/L, respectively.The mole ratios of nitrate production to ammonium reduced (MRNN) of these three parallel systems were reduced from initial 69.78%, 72.43% and 64.67% to 19.91%, 41.02% and 32.96%, respectively. After dosing N2H4 stopped, the nitrate build-up was observed again in 2#SBR. The SVI values of sludge in all these three SBRs were gradually increased and the filaments were developed after restoration. (3) In the case of successful in-situ restoration of the NOB problem in CPNA process by dosing NH2OH, the abundances of the ammonium oxidation encoding genes amoA, hao and anammox encoding gene hzo were increased to 26.58%, 43.56% and 26.17%, respectively. The diversity index of microbial community was reduced, but the abundance of filaments was increased. The filament might act as the denitrifyer, reducing the nitrate to nitrite, which was the substrate of anammox bacteria. Therefore, with the cooperation between the direact inhibition of NOB by dosing NH2OH and the proliferation of the filaments, the nitrate build-up and high NOB activity were greatly relieved, thus the nitrogen removal could be in-situ recovered.
内容类型: 学位论文
URI标识: http://ir.rcees.ac.cn/handle/311016/38710
Appears in Collections:环境水质学国家重点实验室_学位论文

Files in This Item:
File Name/ File Size Content Type Version Access License
王元月--羟胺和肼调控一体式部分亚硝化-厌氧氨氧化工艺的对比研究.pdf(4429KB)学位论文--限制开放 联系获取全文

作者单位: 1.中国科学院生态环境研究中心

Recommended Citation:
王元月. 羟胺和肼调控一体式部分亚硝化-厌氧氨氧化工艺的对比研究[D]. 北京. 中国科学院大学. 2017.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[王元月]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[王元月]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院生态环境研究中心 - Feedback
Powered by CSpace