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Chronic impacts of oxytetracycline on mesophilic anaerobic digestion of excess sludge: Inhibition of hydrolytic acidification and enrichment of antibiotic resistome
Tian, Zhe; Zhang, Yu; Yang, Min
2018-07-01
Source PublicationENVIRONMENTAL POLLUTION
ISSN0269-7491
Volume238Pages:1017-1026
SubtypeArticle
AbstractWe evaluated the chronic impact of oxytetracycline (OTC) on performance and antibiotic resistance development during the mesophilic anaerobic digestion (AD) of antibiotic-containing biomass. Mesophilic AD was conducted in a completely stirred tank reactor by constantly feeding municipal excess sludge spiked with increasing concentrations of OTC (0-1000 mg L-1) under a solid retention time of 20 days over a period of 265 days. Results showed that methane generation of mesophilic AD was inhibited when the OTC concentration in digested sludge was increased to around 18,000 mg k(-1) (OTC dose, 1000 mg L-1), due to the inhibition of fermenting and acidogenic bacteria. Metagenomic sequencing and high-throughput quantitative PCR analysis demonstrated that tetracycline resistance genes were the most dominant type (38.47-43.76%) in the resistome, with tetG, tetX, tetM, tetR, tetQ, tetO, and tetL as the dominant resistant subtypes throughout the whole experimental period. The relative abundance of these tet genes increased from 2.10 x 10(-1) before spiking OTC (OTC concentration in digested sludge, 8.97 mg kg(-1)) to 2.83 x 10(-1) (p < 0.05) after spiking OTC at a dose of 40 mg L-1 (OTC concentration in digested sludge, 528.52 mg kg(-1)). Furthermore, mobile genetic elements, including integrons, transposons, and plasmids, were also enriched with the increase in OTC dose. Based on partial canonical correspondence analysis, the contributions of horizontal (mobile element alteration) and vertical (bacterial community shift) gene transfer to antibiotic resistome variation were 29.35% and 21.51%, respectively. Thus, considering the inhibition of hydrolytic acidification and enrichment of antibiotic resistome, mesophilic AD is not suggested to directly treat the biomass containing OTC concentration higher than 200 mg L-1. (C) 2018 Elsevier Ltd. All rights reserved.
Department环境水质学国家重点实验室
KeywordMesophilic anaerobic digestion Oxytetracycline Hydrolytic acidification Antibiotic resistance genes High-throughput quantitative PCR
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/41121
Collection环境水质学国家重点实验室
Recommended Citation
GB/T 7714
Tian, Zhe,Zhang, Yu,Yang, Min. Chronic impacts of oxytetracycline on mesophilic anaerobic digestion of excess sludge: Inhibition of hydrolytic acidification and enrichment of antibiotic resistome[J]. ENVIRONMENTAL POLLUTION,2018,238:1017-1026.
APA Tian, Zhe,Zhang, Yu,&Yang, Min.(2018).Chronic impacts of oxytetracycline on mesophilic anaerobic digestion of excess sludge: Inhibition of hydrolytic acidification and enrichment of antibiotic resistome.ENVIRONMENTAL POLLUTION,238,1017-1026.
MLA Tian, Zhe,et al."Chronic impacts of oxytetracycline on mesophilic anaerobic digestion of excess sludge: Inhibition of hydrolytic acidification and enrichment of antibiotic resistome".ENVIRONMENTAL POLLUTION 238(2018):1017-1026.
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