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Analysis of silver-associated proteins in pathogen via combination of native SDS-PAGE, fluorescent staining, and inductively coupled plasma mass spectrometry
Li, Yiling; Zhang, Mengni1; Zheng, Chengbin1; Hu, Ligang2; Wang, Chao3; Jiang, Jie3; He, Bin3; Jiang, Guibin
2019-12-06
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1607Pages:-
AbstractCharacterization of silver-associated proteins is important to elucidate underlined mechanisms of silver-containing materials against microbes. Gel electrophoresis based methods are the most popular and basic strategy for the analysis of biomolecules, i.e., proteins and nucleic acids. It solely provides molecular weights of analytes. Extending the method from molecular weight to elemental composition is highly desired when investigating metal-containing molecules. Herein, a gel electrophoresis based method combining native sodium dodecyl sulfate-polyacrylamide gel electrophoresis (native SDS-PAGE), fluorescent staining, and inductively coupled plasma mass spectrometry (ICP-MS) strategy was developed for separation and detection of silver-associated proteins. Two home-made silver-labeled proteins, carbonic anhydrase and ovalbumin, were used for validation of the strategy performance. Silver-associated proteins in Pseudomonas aeruginosa and Staphylococcus aureus treated with silver nanoparticles were further characterized by this method. Some well-known and new proteins were identified to associate to silver in both P. aeruginosa and S. aureus, demonstrating the feasibility of the developed strategy. In conclusion, the current study provides a convenient method for readily identification of silver-associated proteins in biological samples. (C) 2019 Elsevier B.V. All rights reserved.
Department环境化学与生态毒理学国家重点实验室
KeywordNative SDS-PAGE Fluorescent staining ICP-MS Silver-associated proteins Microbes
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/42507
Collection环境化学与生态毒理学国家重点实验室
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Sichuan Univ, Coll Chem, Minist Educ, Key Lab Green Chem & Technol, Chengdu 610064, Sichuan, Peoples R China
3.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China
4.Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Li, Yiling,Zhang, Mengni,Zheng, Chengbin,et al. Analysis of silver-associated proteins in pathogen via combination of native SDS-PAGE, fluorescent staining, and inductively coupled plasma mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2019,1607:-.
APA Li, Yiling.,Zhang, Mengni.,Zheng, Chengbin.,Hu, Ligang.,Wang, Chao.,...&Jiang, Guibin.(2019).Analysis of silver-associated proteins in pathogen via combination of native SDS-PAGE, fluorescent staining, and inductively coupled plasma mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1607,-.
MLA Li, Yiling,et al."Analysis of silver-associated proteins in pathogen via combination of native SDS-PAGE, fluorescent staining, and inductively coupled plasma mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1607(2019):-.
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