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In situ calibration of Direct Analysis in Real Time-mass spectrometry for direct quantification: Urine excretion rate index creatinine as an example
Zhang, Ning; Lu, Meiling1; Duan, Xiaokun; Liu, Charles C.; Wang, Hailin3
2019-08-15
Source PublicationTALANTA
ISSN0039-9140
Volume201Pages:134-142
AbstractAmbient ionization in open environment brings a capability of a coupled mass spectrometry to detect target molecules in situ. However, it is limited to qualitative and semi-quantitative analysis. By coupling of an ambient ionization-based Direct Analysis in Real Time (DART) with high-resolution quadrupole time-of-flight mass spectrometry (QTOF/MS), we observe that, in one-chemical system, the target molecule displays a non-linear response in MS signal vs concentration, accompanying with large variation in MS signal, suggesting two obstacles for quantification to be overcome. Surprisingly, in a two-chemical system, we observe an apparent suppression effect. We prove that, due to this observed suppression effect, a fluctuant response in the MS signal of the stable isotope-labeled analogue can immediately reflect the change in the analyte concentration and ionization efficiency. For example, by taking advantage of this effect, even the analyte of different concentrations despairingly displayed similar signals would be accurately calibrated through the suppression of the internal stable isotope standard. This puts an important foundation on accurate and linear quantitation of analytes in complex matrix using DART-MS assay. Moreover, we for the first time demonstrate an application of in situ calibration of DART-MS for direct and accurate quantification of target molecule (creatinine) in highly complex samples (human urine) without any pre-separation. The quantification is also validated using HPLC-UV analysis (n = 38). At last, we show that stable isotope-labeled-creatinine (m/z 117.0850 amu) can be used for simultaneous in situ calibration of some other urinary metabolites with a mass/charge ratio varying from 120.069 amu to 333.125 amu.
Department环境化学与生态毒理学国家重点实验室
KeywordDirect analysis in real time Direct quantification Urinary creatinine Stable isotope-labeled creatinine
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/42551
Collection环境化学与生态毒理学国家重点实验室
Affiliation1.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Agilent Technol, Chem Anal Grp, Beijing 100102, Peoples R China
3.Duan, Xiaokun; Liu, Charles C.] ASPEC Technol Ltd, Beijing 100101, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Ning,Lu, Meiling,Duan, Xiaokun,et al. In situ calibration of Direct Analysis in Real Time-mass spectrometry for direct quantification: Urine excretion rate index creatinine as an example[J]. TALANTA,2019,201:134-142.
APA Zhang, Ning,Lu, Meiling,Duan, Xiaokun,Liu, Charles C.,&Wang, Hailin.(2019).In situ calibration of Direct Analysis in Real Time-mass spectrometry for direct quantification: Urine excretion rate index creatinine as an example.TALANTA,201,134-142.
MLA Zhang, Ning,et al."In situ calibration of Direct Analysis in Real Time-mass spectrometry for direct quantification: Urine excretion rate index creatinine as an example".TALANTA 201(2019):134-142.
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