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Scattered Light Imaging Enables Real-Time Monitoring of Label-Free Nanoparticles and Fluorescent Biomolecules in Live Cells
Wang, Fengbang1,5; Chen, Bolei2; Yan, Bing3; Yin, Yongguang1; Hu, Ligang; Liang, Yong2,4; Song, Maoyong1,5; Jiang, Guibin5
2019-09-11
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume141Issue:36Pages:14043-14047
AbstractSimultaneously monitoring label-free nanoparticles (NPs) and fluorescent biomolecules inside the live cell in real time is challenging because both imaging methods require different instrumentation and measuring principles. Here we report a novel scattered light imaging (SLi) technique that allows label-free NPs to be monitored using a conventional confocal microscope. The method shows a high spatial resolution and can distinguish label-free silver nanoparticles (AgNPs) with a 10 nm size difference in live cells. We performed SLi to observe the uptake, movement, distribution, and transformation of AgNPs in live cells at a single-particle level. The method is applicable to accurately track the localization of a variety of nanomaterials inside the cell. With this approach, label-free NP and fluorescent-labeled biomolecules are imaged simultaneously making it possible to real-time monitor nanobio interactions.
Department环境化学与生态毒理学国家重点实验室
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/42585
Collection环境化学与生态毒理学国家重点实验室
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Environm Nanotechnol & Hlth Effects, Beijing 100085, Peoples R China
3.Jianghan Univ, Hubei Key Lab Environm & Hlth Effects Persistent, Wuhan 430056, Hubei, Peoples R China
4.Guangzhou Univ, Minist Educ, Key Lab Water Qual & Conservat Pearl River Delta, Inst Environm Res Greater Bay, Guangzhou 510006, Guangdong, Peoples R China
5.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Fengbang,Chen, Bolei,Yan, Bing,et al. Scattered Light Imaging Enables Real-Time Monitoring of Label-Free Nanoparticles and Fluorescent Biomolecules in Live Cells[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2019,141(36):14043-14047.
APA Wang, Fengbang.,Chen, Bolei.,Yan, Bing.,Yin, Yongguang.,Hu, Ligang.,...&Jiang, Guibin.(2019).Scattered Light Imaging Enables Real-Time Monitoring of Label-Free Nanoparticles and Fluorescent Biomolecules in Live Cells.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,141(36),14043-14047.
MLA Wang, Fengbang,et al."Scattered Light Imaging Enables Real-Time Monitoring of Label-Free Nanoparticles and Fluorescent Biomolecules in Live Cells".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 141.36(2019):14043-14047.
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