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Developmental dynamics of antibiotic resistome in aerobic biofilm microbiota treating wastewater under stepwise increasing tigecycline concentrations
Tian, Zhe; Liu, Ruyin; Zhang, Hong; Yang, Min; Zhang, Yu
2019-10-01
Source PublicationENVIRONMENT INTERNATIONAL
ISSN0160-4120
Volume131Pages:-
AbstractThis study aimed to investigate the impact of tigecycline, the third generation tetracycline, on the antibiotic resistance development in environmental microbiota. Two biological contact oxidation reactors containing aerobic biofilm microbiota were constructed, one of which was constantly fed with synthetic wastewater spiked with increasing concentrations of tigecycline (0 to 25 mg/L) under a hydrolytic retention time of 24 h. Over a period of 636 days, chemical oxygen demand removal over 90% and complete nitrification were achieved for both the control and tigecycline-exposed reactors, and effluent tigecycline concentrations in the tigecycline-exposed system were always < 0.051 mg/L. Significant increases (p < 0.01) in resistome abundance and resistant bacteria ratio were detected at a tigecycline dose of 10 and 25 mg/L, respectively, revealed by metagenomic sequencing and culture-based method. The increase of resistome in the tigecycline system was mainly attributed to the enrichment of tetX, one cooperative tetracycline degrading gene. Partial canonical correspondence analysis showed that the change of resistome was mainly driven by bacterial community shift (vertical pathway). Network and genome binning analyses further suggested that the proliferation of Flavobacterium harboring tetX contributed to a relatively low community-wide resistance development in the aerobic biofilm microbiota under tigecycline selection by reducing the antibiotic concentration. This work provides scientific bases for the management and evaluation of the resistance risk induced by this novel antibiotic.
Department环境水质学国家重点实验室
KeywordDynamics Resistome Biofilm microbiota Tigecycline Metagenomic sequencing
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/42638
Collection环境水质学国家重点实验室
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Natl Engn Lab Ind Wastewater Treatment, Beijing 100085, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Tian, Zhe,Liu, Ruyin,Zhang, Hong,et al. Developmental dynamics of antibiotic resistome in aerobic biofilm microbiota treating wastewater under stepwise increasing tigecycline concentrations[J]. ENVIRONMENT INTERNATIONAL,2019,131:-.
APA Tian, Zhe,Liu, Ruyin,Zhang, Hong,Yang, Min,&Zhang, Yu.(2019).Developmental dynamics of antibiotic resistome in aerobic biofilm microbiota treating wastewater under stepwise increasing tigecycline concentrations.ENVIRONMENT INTERNATIONAL,131,-.
MLA Tian, Zhe,et al."Developmental dynamics of antibiotic resistome in aerobic biofilm microbiota treating wastewater under stepwise increasing tigecycline concentrations".ENVIRONMENT INTERNATIONAL 131(2019):-.
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