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RNA Stable Isotope Probing of Potential Feammox Population in Paddy Soil
Li, Hu; Su, Jian-Qiang; Yang, Xiao-Ru; Zhou, Guo-Wei; Lassen, Simon Bo; Zhu, Yong-Guan
2019-05-07
Source PublicationENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN0013-936X
Volume53Issue:9Pages:4841-4849
AbstractAnaerobic ammonium oxidation coupled to iron reduction (Feammox) is a recently discovered pathway contributing to nitrogen loss in various ecosystems such as paddy soils and sediments. However, little is known about the microbes driving Feammox in an agricultural ecosystem. Here, we demonstrated the occurrence of Feammox in paddy soils of Southern China using a N-15 isotopic tracing technique, and examined the microbial communities associated with Feammox using RNA based stable isotope probing (RNA-SIP) combined with Illumina sequencing. Feammox was detected in all collected soils with direct N-2 production as the dominant Feammox pathway. It was estimated that approximately 6.91% of the applied nitrogen fertilizers were lost through Feammox in the paddy soils. RNA-SIP results showed that the composition of enriched active microbial communities were dependent on soil properties, especially the soil pH and grain size. Geobacter were enriched in most soils across various properties. The abundance of enriched GOUTA19 were significantly higher in soils with low pH than those in soils with medium pH and high pH, and the relative abundance of active Nitrososphaeraceae and Pseudomonas only increased in soils with medium and high pH during 4-day of incubation. These results suggested Feammox is a ubiquitous and important process for N loss. Geobacter, GOUTA19, Nitrososphaeraceae and Pseudomonas were active during the incubation that favored Feammox and the growth of Feammox microbes, suggesting these microbes were potentially associated with Feammox in natural agricultural soils.
Department城市与区域生态国家重点实验室
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/42785
Collection城市与区域生态国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China
2.Chinese Acad Sci, Inst Urban Environm, Ctr Excellence Reg Atmospher Environm, Xiamen 361021, Peoples R China
3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China
4.Univ Copenhagen, Fac Sci, Dept Plant & Environm Sci, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark
Recommended Citation
GB/T 7714
Li, Hu,Su, Jian-Qiang,Yang, Xiao-Ru,et al. RNA Stable Isotope Probing of Potential Feammox Population in Paddy Soil[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2019,53(9):4841-4849.
APA Li, Hu,Su, Jian-Qiang,Yang, Xiao-Ru,Zhou, Guo-Wei,Lassen, Simon Bo,&Zhu, Yong-Guan.(2019).RNA Stable Isotope Probing of Potential Feammox Population in Paddy Soil.ENVIRONMENTAL SCIENCE & TECHNOLOGY,53(9),4841-4849.
MLA Li, Hu,et al."RNA Stable Isotope Probing of Potential Feammox Population in Paddy Soil".ENVIRONMENTAL SCIENCE & TECHNOLOGY 53.9(2019):4841-4849.
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