RCEES OpenIR  > 环境化学与生态毒理学国家重点实验室
3D printed gel electrophoresis device coupling with ICP-MS for online separation and detection of metalloproteins
Wang, Dingyi; He, Bin; Yan, Xueting; Nong, Qiying; Wang, Chao; Jiang, Jie; Hu, Ligang; Jiang, Guibin
2019-05-15
Source PublicationTALANTA
ISSN0039-9140
Volume197Pages:145-150
AbstractWe successfully developed a strategy to combine a customized gel electrophoresis device with ICP-MS for online separation and detection of metalloproteins. The self-designed horizontal column gel electrophoresis device was rapidly and easily fabricated in the laboratory via 3D printing with a low cost. The feasibility of 3D printing to fabricate this device was investigated by offline separation of commercial protein standards. And a better separation efficiency was found when using gel tubes printed with higher printing precision. As a proof-of-concept, the performance of the whole system is demonstrated by online separation and detection of both iodinated protein standards and proteins in rat blood plasma samples. Benefits from 3D printing, customized modification or further optimization can be readily achieved for a better protein separation and detection efficiency.
Department环境化学与生态毒理学国家重点实验室
Keyword3D printing Gel electrophoresis Metalloprotein ICP-MS
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/42882
Collection环境化学与生态毒理学国家重点实验室
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China
3.Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Peoples R China
4.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Dingyi,He, Bin,Yan, Xueting,et al. 3D printed gel electrophoresis device coupling with ICP-MS for online separation and detection of metalloproteins[J]. TALANTA,2019,197:145-150.
APA Wang, Dingyi.,He, Bin.,Yan, Xueting.,Nong, Qiying.,Wang, Chao.,...&Jiang, Guibin.(2019).3D printed gel electrophoresis device coupling with ICP-MS for online separation and detection of metalloproteins.TALANTA,197,145-150.
MLA Wang, Dingyi,et al."3D printed gel electrophoresis device coupling with ICP-MS for online separation and detection of metalloproteins".TALANTA 197(2019):145-150.
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