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Synthesis and purification of biotinylated oligodeoxynucleotides containing single TpT dimeric pyrimidine (6-4) pyrimidone lesion
Wu, Danni; Zhang, Ning; Kong, Bingjie; Hang, Haiying; Wang, Hailin
2019-07
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
Volume411Issue:18Pages:4123-4129
AbstractUltraviolet (UV) radiation could induce pyrimidine-related dimeric lesions in genomic DNA. Though the cyclobutane pyrimidine dimers (CPDs) are the most abundant UV-induced lesions, the pyrimidine (6-4) pyrimidone photoproducts (6-4PPs) may have more serious, potentially lethal, and mutagenic effects. It is important to have 6-4PP-containing oligodeoxynucleotides to be prepared for studying their adverse biological effects. Here, we developed a UV-irradiated water droplet method for the preparation of a biotinylated, 6-4PP-containing 10-mer oligodeoxynucleotide. By the use of HPLC purification and enrichment twice, the final yield is estimated to be about 8.1%. In contrast, without applying droplet technique, the direct UV irradiation against oligonucleotide-containing aqueous solution, the product yield is very low. The enzymatic hydrolyzation of the obtained product shows a 6-4PP characteristic ion transition of 545.12 -> 432.13 in negative ion mode UHPLC-Q-TOF/MS. The established procedure for the preparation of 6-4PP-containing oligonucleotides is convenient with an improved yield.
Department环境化学与生态毒理学国家重点实验室
KeywordUV irradiation Pyrimidine (6-4) pyrimidone (6-4PP) HPLC UHPLC-Q-TOF/MS
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/43029
Collection环境化学与生态毒理学国家重点实验室
Affiliation1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Key Lab Prot & Peptide Pharmaceut, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wu, Danni,Zhang, Ning,Kong, Bingjie,et al. Synthesis and purification of biotinylated oligodeoxynucleotides containing single TpT dimeric pyrimidine (6-4) pyrimidone lesion[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(18):4123-4129.
APA Wu, Danni,Zhang, Ning,Kong, Bingjie,Hang, Haiying,&Wang, Hailin.(2019).Synthesis and purification of biotinylated oligodeoxynucleotides containing single TpT dimeric pyrimidine (6-4) pyrimidone lesion.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(18),4123-4129.
MLA Wu, Danni,et al."Synthesis and purification of biotinylated oligodeoxynucleotides containing single TpT dimeric pyrimidine (6-4) pyrimidone lesion".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.18(2019):4123-4129.
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