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The brominated flame retardant PBDE 99 promotes adipogenesis via regulating mitotic clonal expansion and PPAR gamma expression
Wen, Qing; Xie, Xinni; Zhao, Chuanfang; Ren, Qidong; Zhang, Xinyi; Wei, Dongbin; Emanuelli, Brice; Du, Yuguo
2019-06-20
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
Volume670Pages:67-77
AbstractObesogens have been widely accepted as chemicals that promote obesity, and there are many environmental pollutants that were functionally identified as obesogens. PBDE 99 is one of the most abundant PBDE congeners detected in human. However, its obesogenic effects are poorly understood. Here, we explore the in vitro effects of PBDE 99 on adipogenesis, which is a key process in obesogenesis. We observed an increase in adipogenesis when differentiating cells were exposed to PBDE 99. Further, the promoting effects of PBDE 99 on adipogenesis were most efficient during the first 4 days of 313-L1 differentiation. Consistent with this, early transcriptional factor CCAAT/enhancer-binding proteins beta (C/EBP beta) was upregulated at Days 1 and 2 during differentiation, which is accompanied with the acceleration of mitotic clonal expansion (MCE) and the upregulation of terminal transcriptional factors C/EBP alpha and PPAR gamma 2 from Day 2 or Day 4. Additionally, bisulfite genomic sequencing analysis revealed that PBDE 99 decreased methylation status of the CpG sites at PPAR gamma promoter region. Collectively, these findings demonstrate that PBDE 99 may be a potential environmental obesogen by promoting adipogenesis through facilitating MCE progression at early differentiation stage and upregulating key adipogenic factor PPAR gamma 2 expression both in direct transcriptional and epigenetic regulation dependent manner. (C) 2019 Elsevier B.V. All rights reserved.
Department环境化学与生态毒理学国家重点实验室
Keyword3T3-L1 C/EBP beta Epigenetic modification Obesogen
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/43036
Collection环境化学与生态毒理学国家重点实验室
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn, Ctr Basic Metab Res, Copenhagen, Denmark
Recommended Citation
GB/T 7714
Wen, Qing,Xie, Xinni,Zhao, Chuanfang,et al. The brominated flame retardant PBDE 99 promotes adipogenesis via regulating mitotic clonal expansion and PPAR gamma expression[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,670:67-77.
APA Wen, Qing.,Xie, Xinni.,Zhao, Chuanfang.,Ren, Qidong.,Zhang, Xinyi.,...&Du, Yuguo.(2019).The brominated flame retardant PBDE 99 promotes adipogenesis via regulating mitotic clonal expansion and PPAR gamma expression.SCIENCE OF THE TOTAL ENVIRONMENT,670,67-77.
MLA Wen, Qing,et al."The brominated flame retardant PBDE 99 promotes adipogenesis via regulating mitotic clonal expansion and PPAR gamma expression".SCIENCE OF THE TOTAL ENVIRONMENT 670(2019):67-77.
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