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Evaluation of the secondary structures of protein in the extracellular polymeric substances extracted from activated sludge by different methods
Xiao, Benyi; Liu, Yu; Luo, Meng; Yang, Tang; Guo, Xuesong; Yi, Hao
2019-06
Source PublicationJOURNAL OF ENVIRONMENTAL SCIENCES
ISSN1001-0742
Volume80Pages:128-136
AbstractThe changes of protein secondary structures in the extracellular polymeric substances (EPS) extracted from activated sludge by four different methods were studied by analyzing the amide I region (1700-1600 cm(-1)) of the Fourier transform infrared spectra and model protein test. The results showed the molecular weight distribution of organic matter extracted by centrifugation, heating and cation exchange resin (CER) was similar, while the EPS extracted by centrifugation (Control) and CER had similar fluorescent organic matter. The protein secondary structures of extracted EPS by the four methods were different. The similarities of protein secondary structures between the EPS extracted by CER with the Control were the highest among the four extracted EPS. Although the EPS yield extracted by formaldehyde + NaOH method were the highest, its protein secondary structures had the lowest similarity with those extracted by the Control. Additionally, the effects of centrifugation and CER extraction on the secondary structures of bovine serum albumin were also lower than that of other extraction processes. CER enables the second maximum extraction of EPS and maximum retention of the original secondary structure of proteins. (C) 2018 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
Department水污染控制实验室
KeywordActivated sludge Different extraction methods Extracellular polymeric substances Protein secondary structure
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/43246
Collection水污染控制实验室
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Beijing ZEHO Waterfront Ecol Environm Treatment C, Beijing 100085, Peoples R China
4.Minist Environm Protect, South China Inst Environm Sci, Guangzhou 510655, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Xiao, Benyi,Liu, Yu,Luo, Meng,et al. Evaluation of the secondary structures of protein in the extracellular polymeric substances extracted from activated sludge by different methods[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2019,80:128-136.
APA Xiao, Benyi,Liu, Yu,Luo, Meng,Yang, Tang,Guo, Xuesong,&Yi, Hao.(2019).Evaluation of the secondary structures of protein in the extracellular polymeric substances extracted from activated sludge by different methods.JOURNAL OF ENVIRONMENTAL SCIENCES,80,128-136.
MLA Xiao, Benyi,et al."Evaluation of the secondary structures of protein in the extracellular polymeric substances extracted from activated sludge by different methods".JOURNAL OF ENVIRONMENTAL SCIENCES 80(2019):128-136.
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