RCEES OpenIR  > 中国科学院饮用水科学与技术重点实验室
Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples
Liu, Lanhua; Zhou, Xiaohong; Lu, Yun; Shi, Hanchang; Ma, Mei; Yu, Tong
2019-11-01
Source PublicationENVIRONMENT INTERNATIONAL
ISSN0160-4120
Volume132Pages:-
AbstractEstablishing biosensors to map a comprehensive picture of potential estrogen-active chemicals remains challenging and must be addressed. Herein, we describe an estrogen receptor (ER)-based evanescent wave fluorescent biosensor by using a triple functional small-molecule-protein conjugate as a signal probe for the determination of estrogenic activities in water samples. The signal probe, consisting of a Cy5.5-labelled streptavidin (STV) moiety and a 17 beta-estradiol (E-2) moiety, acts simultaneously as signal conversion, signal recognition and signal report elements. When xenoestrogens compete with the E-2 moiety of conjugate in binding to the ER, the unbound conjugates are released, and their STV moiety binds with desthiobiotin (DTB) modified on the optical fiber via the STV-DTB affinity interactions. Signal probe detection is accomplished by fluorescence emission induced by an evanescent field, which positively relates with the estrogenic activities in samples. Quantification of estrogenic activity expressed as E-2 equivalent concentration (EEQ) can be achieved with a detection limit of 1.05 mu g/L EEQ by using three times standard deviation of the mean blank values and a linear calibration range from 20.8 to 476.7 mu g/L EEQ. The optical fiber system is robust enough for hundreds of sensing cycles. The biosensor-based determination of estrogenic activities in wastewater samples obtained from a full-scale wastewater treatment plant is consistent with that measured by the two-hybrid recombinant yeast bioassay.
Department中国科学院饮用水科学与技术重点实验室
KeywordEstrogen receptor Small-molecule-protein conjugate Optical fiber biosensor 17 beta-estradiol equivalent concentration Wastewater sample
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/43544
Collection中国科学院饮用水科学与技术重点实验室
Affiliation1.Tsinghua Univ, Sch Environm, State Key Joint Lab ESPC, Beijing 100084, Peoples R China
2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China
3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Lanhua,Zhou, Xiaohong,Lu, Yun,et al. Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples[J]. ENVIRONMENT INTERNATIONAL,2019,132:-.
APA Liu, Lanhua,Zhou, Xiaohong,Lu, Yun,Shi, Hanchang,Ma, Mei,&Yu, Tong.(2019).Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples.ENVIRONMENT INTERNATIONAL,132,-.
MLA Liu, Lanhua,et al."Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples".ENVIRONMENT INTERNATIONAL 132(2019):-.
Files in This Item: Download All
File Name/Size DocType Version Access License
Triple functional sm(1006KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Lanhua]'s Articles
[Zhou, Xiaohong]'s Articles
[Lu, Yun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Lanhua]'s Articles
[Zhou, Xiaohong]'s Articles
[Lu, Yun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Lanhua]'s Articles
[Zhou, Xiaohong]'s Articles
[Lu, Yun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.