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Heterogenous Internalization of Nanoparticles at Ultra-Trace Concentration in Environmental Individual Unicellular Organisms Unveiled by Single-Cell Mass Cytometry
Wu, Qi; Shi, Jianbo; Ji, Xiaomeng; Xia, Tian; Zeng, Li; Li, Gengtan; Wang, Yuanyuan; Gao, Jie; Yao, Linlin; Ma, Junjie; Liu, Xiaolei; Liu, Nian; Hu, Ligang; He, Bin; Liang, Yong; Qu, Guangbo; Jiang, Guibin
2020-10-27
Source PublicationACS NANO
ISSN1936-0851
Volume14Issue:10Pages:12828-12839
AbstractThe application and consumption of nano particles (NPs) inevitably result in the contamination of environmental water. The internalized NPs in unicellular organisms could travel to human bodies along food chains and raise health concerns. Current research failed to determine the characteristics of cellular uptake of NPs by unicellular organisms at extremely low concentration in the real environment. We here developed a label-free high-throughput mass cytometry method to investigate gold NP (AuNP) uptake in a unicellular organism (Tetrahymena thermophila) at the single-cell level. The limit of detection for Au is as low as to 6.67 x 10(-8) g/cell, which equals similar to 5.3 5 nm AuNPs. We demonstrated that active engulfment pathways were responsible for the cellular accumulation of AuNPs and T. thermophila could also eliminate the cellular AuNPs rapidly. The interaction between AuNPs and T. thermophila is highly dependent on the sizes of nanoparticles; i.e., the population of T. thermophila containing AuNPs decreased with the increment of the diameters of AuNPs when exposed to the same mass concentration. For each type of AuNP, distinct heterogeneous cellular uptake of AuNPs by T. thermophila was observed. Intriguingly, for 5 nm AuNP, even at 0.001 ng/mL, some T. thermophila cells could concentrate AuNPs, indicating a real environmental concern even when water was contaminated by only trace level of NPs. This method represents a promising tool for simultaneous determination of physiological status of cells together with the intracellular level of heavy metal or metallic NPs in study of biological effects.
Department环境化学与生态毒理学国家重点实验室
Keywordmass cytometry single cell trace level nanoparticle bioconcentration
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/44703
Collection环境化学与生态毒理学国家重点实验室
Recommended Citation
GB/T 7714
Wu, Qi,Shi, Jianbo,Ji, Xiaomeng,et al. Heterogenous Internalization of Nanoparticles at Ultra-Trace Concentration in Environmental Individual Unicellular Organisms Unveiled by Single-Cell Mass Cytometry[J]. ACS NANO,2020,14(10):12828-12839.
APA Wu, Qi.,Shi, Jianbo.,Ji, Xiaomeng.,Xia, Tian.,Zeng, Li.,...&Jiang, Guibin.(2020).Heterogenous Internalization of Nanoparticles at Ultra-Trace Concentration in Environmental Individual Unicellular Organisms Unveiled by Single-Cell Mass Cytometry.ACS NANO,14(10),12828-12839.
MLA Wu, Qi,et al."Heterogenous Internalization of Nanoparticles at Ultra-Trace Concentration in Environmental Individual Unicellular Organisms Unveiled by Single-Cell Mass Cytometry".ACS NANO 14.10(2020):12828-12839.
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