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High-Throughput Single Cell Analysis Reveals the Heterogeneity of QDots-Induced Response in Macrophages
Wang, Yuanyuan; Hu, Ligang; Xia, Tian; Yao, Xinglei; Yin, Yongguang; Wang, Fengbang; Song, Maoyong; Jiang, Guibin
2020-05-12
Source PublicationENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
ISSN2328-8930
Volume7Issue:5Pages:337-342
AbstractThe ability to assess the responses of cells exposed to nanoparticles at the single cell level will lead to mechanistic understanding of their toxicity and safety. Here, we demonstrated the heterogeneity of cell uptake and cellular responses in a QDots-exposed macrophage using a high-content screening (HCS) analysis. The dynamic uptake and intracellular location of QDots were characterized by HCS due to its fluorescence. We further verified the association between fluorescence intensity and mass concentration of intracellular QDots using combined methods. The heterogeneity of multiple cell responses, including apoptosis, autophagy, calcium influx, mitochondria membrane potential, nucleus size, and reactive oxygen species generation, was analyzed for fingerprinting of the cytotoxicity of QDots at the single cell level. On the basis of the heterogeneity and the established dose-response relationship, we illustrated that ROS generation was the most sensitive parameter to intracellular QDots. This study makes an important methodological contribution to nanotoxicology with much higher throughput and accuracy at the single cell level.
Department环境纳米技术与健康效应重点实验室
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/44875
Collection环境纳米技术与健康效应重点实验室
Recommended Citation
GB/T 7714
Wang, Yuanyuan,Hu, Ligang,Xia, Tian,et al. High-Throughput Single Cell Analysis Reveals the Heterogeneity of QDots-Induced Response in Macrophages[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS,2020,7(5):337-342.
APA Wang, Yuanyuan.,Hu, Ligang.,Xia, Tian.,Yao, Xinglei.,Yin, Yongguang.,...&Jiang, Guibin.(2020).High-Throughput Single Cell Analysis Reveals the Heterogeneity of QDots-Induced Response in Macrophages.ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS,7(5),337-342.
MLA Wang, Yuanyuan,et al."High-Throughput Single Cell Analysis Reveals the Heterogeneity of QDots-Induced Response in Macrophages".ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS 7.5(2020):337-342.
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