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Transcriptional and metabolic response against hydroxyethane-(1,1-bisphosphonic acid) on bacterial denitrification by a halophilic Pannonibacter sp. strain DN
Koju, Rashmi; Miao, Shiyu; Liang, Bin; Joshi, Dev Raj; Bai, Yaohui; Liu, Ruiping; Qu, Juihui
2020-08
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume252Pages:1-11
AbstractBiological denitrification is an environmentally sound pathway for the elimination of nitrogen pollution in wastewater treatment. Extreme environmental conditions, such as the co-existence of toxic organic pollutants, can affect biological denitrification. However, the potential underlying mechanism remains largely unexplored. Herein, the effect of a model pollutant, hydroxyethane-(1,1-bisphosphonic acid) (HEDP), a widely applied and consumed bisphosphonate, on microbial denitrification was investigated by exploring the metabolic and transcriptional responses of an isolated denitrifier, Pannonibacter sp. strain DN. Results showed that nitrate removal efficiency decreased from 85% to 50% with an increase in HEDP concentration from 0 to 3.5 mM, leading to nitrite accumulation of 204 mg L-1 in 3.5 mM HEDP. This result was due to the lower bacterial population count and reduction in the live cell percentage. Further investigation revealed that HEDP caused a decrease in membrane potential from 0.080 +/- 0.005 to 0.020 +/- 0.002 with the increase in HEDP from 0 to 3.5 mM. This hindered electron transfer, which is required for nitrate transformation into nitrogen gas. Moreover, transcriptional profiling indicated that HEDP enhanced the genes involved in ROS (O-2(-)) scavenging, thus protecting cells against oxidative stress damage. However, the suppression of genes responsible for the production of NADH/FADH(2) in tricarboxylic acid cycle (TCA), NADH catalyzation (NADH dehydrogenase) in (electron transport chain) ETC system and denitrifying genes, especially nor and nir, in response to 2.5 mM HEDP were identified as the key factor inhibiting transfer of electron from TCA cycle to denitrifying enzymes through ETC system. (C) 2020 Elsevier Ltd. All rights reserved.
Department中国科学院饮用水科学与技术重点实验室
KeywordHydroxyethane-(1,1-bisphosphonic acid) Denitrification Pannonibacter sp. DN Membrane potential Transcriptional profiling
Document Type期刊论文
Identifierhttp://ir.rcees.ac.cn/handle/311016/45375
Collection中国科学院饮用水科学与技术重点实验室
Recommended Citation
GB/T 7714
Koju, Rashmi,Miao, Shiyu,Liang, Bin,et al. Transcriptional and metabolic response against hydroxyethane-(1,1-bisphosphonic acid) on bacterial denitrification by a halophilic Pannonibacter sp. strain DN[J]. CHEMOSPHERE,2020,252:1-11.
APA Koju, Rashmi.,Miao, Shiyu.,Liang, Bin.,Joshi, Dev Raj.,Bai, Yaohui.,...&Qu, Juihui.(2020).Transcriptional and metabolic response against hydroxyethane-(1,1-bisphosphonic acid) on bacterial denitrification by a halophilic Pannonibacter sp. strain DN.CHEMOSPHERE,252,1-11.
MLA Koju, Rashmi,et al."Transcriptional and metabolic response against hydroxyethane-(1,1-bisphosphonic acid) on bacterial denitrification by a halophilic Pannonibacter sp. strain DN".CHEMOSPHERE 252(2020):1-11.
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