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Immunomagnetic separation and rapid detection of bacteria using bioluminescence and microfluidics
Qiu, Jingmin; Zhou, Yun; Chen, Hui; Lin, Jin-Ming
2009-08-15
Source PublicationTALANTA
Volume79Issue:3Pages:787-795
AbstractThis paper describes an immunomagnetic separation of target bacterial cells from others by using magnetic bead. The surface of bead was coated with antibodies which can capture specific organism. The binding efficiency of immunomagnetic bead (IMB) capturing target bacterial cells was higher than 98% when the concentrations of target and interferent bacterial cells were at the same level. The concentration of bacteria was determined indirectly by detecting adenosine 5'-triphosphate (ATP) employing bioluminescence (BL) reaction of firefly luciferin-ATR Benzalkonium chloride (BAC) was used as an ATP extractant from living bacterial cells. We found that BAC could enhance the light emission when the concentration of BAC was less than 5.3 x 10(-2)% (w/v) and the BL intensity reached its maximum at the concentration of BAC was 2.7 x 10(-2)%, which was 10-fold stronger than that without BAC. Based on the principle of the IMB, a microfluidic chip combined with immunofluorescence assay for separating and detecting bacteria simultaneously was also developed. The IMBs were magnetically fixed in the bead-beds of chip channels with a 3-mm diameter of NdFeB permanent magnet. The target bacterial cells can be captured magnetically and observed by a fluorescent microscope. (C) 2009 Elsevier B.V. All rights reserved.
Department环境化学与生态毒理学国家重点实验室
KeywordImmunomagnetic Bead Bacteria Salmonella Staphylococcus Aureus Bioluminescence Microfluidics
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Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/21515
Collection环境化学与生态毒理学国家重点实验室
Recommended Citation
GB/T 7714
Qiu, Jingmin,Zhou, Yun,Chen, Hui,et al. Immunomagnetic separation and rapid detection of bacteria using bioluminescence and microfluidics[J]. TALANTA,2009,79(3):787-795.
APA Qiu, Jingmin,Zhou, Yun,Chen, Hui,&Lin, Jin-Ming.(2009).Immunomagnetic separation and rapid detection of bacteria using bioluminescence and microfluidics.TALANTA,79(3),787-795.
MLA Qiu, Jingmin,et al."Immunomagnetic separation and rapid detection of bacteria using bioluminescence and microfluidics".TALANTA 79.3(2009):787-795.
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