| Effect of temperature on the chromatographic behavior of epirubicin and its analogues on high purity silica using reversed-phase solvents | |
| Dong, Lili; Huang, Junxiong | |
| 2007-05 | |
| Source Publication | CHROMATOGRAPHIA
 |
| Volume | 65Issue:1Pages:519-526 |
| Abstract | The influence of temperature on the retention behavior of epirubicin and its analogues on high purity silica with reversed-phase solvents has been systematically investigated. it was found that temperature effects on retention are highly dependent on the type and concentration of organic modifier, as well as the pH of the mobile phase. In organic-rich mobile phases, the type of organic modifier plays an important role. With an aprotic solvent as modifier, retention times show anomalous increases with elevated temperature. At the same time, both efficiency and resolution are significantly improved but this is not the situation with aprotic solvent as modifier. In addition, temperature shows different effects on retention time and selectivity when the pH is changed, and temperature-dependent selectivity reversal is found at higher pHs. In aqueous-rich mobile phases, regardless of the nature of the organic solvent and pH, retention of solutes drops as temperature is raised. It seems that the effect of temperature on chromatographic behavior of the solutes on bare silica using mobile phases containing various organic modifiers or pHs, results from a number of different retention mechanisms. |
| Department | 环境化学与生态毒理学国家重点实验室 |
| Keyword | Column Liquid Chromatography High Purity Silica Reversed-phase Solvents Temperature Effects On retentiOn Van't Hoff Plots |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/22454 |
| Collection | 环境化学与生态毒理学国家重点实验室 |
| Recommended Citation GB/T 7714 | Dong, Lili,Huang, Junxiong. Effect of temperature on the chromatographic behavior of epirubicin and its analogues on high purity silica using reversed-phase solvents[J]. CHROMATOGRAPHIA,2007,65(1):519-526. |
| APA | Dong, Lili,&Huang, Junxiong.(2007).Effect of temperature on the chromatographic behavior of epirubicin and its analogues on high purity silica using reversed-phase solvents.CHROMATOGRAPHIA,65(1),519-526. |
| MLA | Dong, Lili,et al."Effect of temperature on the chromatographic behavior of epirubicin and its analogues on high purity silica using reversed-phase solvents".CHROMATOGRAPHIA 65.1(2007):519-526. |
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