Determination of epirubicin and its impurities in pharmaceutical by hydrophilic interaction chromatography

	Determination of epirubicin and its impurities in pharmaceutical by hydrophilic interaction chromatography
	Li, RP; Dong, LL; Huang, JX
	2005
Source Publication	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
Volume	33 Issue:6 Pages:814-816
Abstract	A novel method for the determination of epirubicin by hydrophilic interaction chromatography (HILIC) using high purity silica column with highly polar organic-aqueous buffer as mobile phase has been developed. The buffer pH and concentration in the mobile phase as well as the flow rate have been optimized. The optimal mobile phase is 90% ( V/V) acetonitrile in 40 mmol/L formate buffer (pH 2.9). The flow rate was 1.0 mL/min. The system suitability has been tested under the optimal chromatographic condition, and the results show that the resolutions between the adjacent components and the asymmetry factors of the solutes meet the pharmacopoeia requirements. The proposed method has good linearity ( r = 0.9971-0.9991) and reproducibility (RSD < 1.0 % for the peak area). It has been proved simple and convenient, and applied in the determination of epirubicin and its impurities in epirubicin raw product with satisfactory results.
Department	环境化学与生态毒理学国家重点实验室
Keyword	Hydrophilic Interaction Chromatography Silica Column Epirubicin
WOS Research Area	Chemistry
Citation statistics
Document Type	期刊论文
Identifier	https://ir.rcees.ac.cn/handle/311016/23162
Collection	环境化学与生态毒理学国家重点实验室
Recommended Citation GB/T 7714	Li, RP,Dong, LL,Huang, JX. Determination of epirubicin and its impurities in pharmaceutical by hydrophilic interaction chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2005,33(6):814-816.
APA	Li, RP,Dong, LL,&Huang, JX.(2005).Determination of epirubicin and its impurities in pharmaceutical by hydrophilic interaction chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,33(6),814-816.
MLA	Li, RP,et al."Determination of epirubicin and its impurities in pharmaceutical by hydrophilic interaction chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 33.6(2005):814-816.

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