Application of preparative chromatography to the isolation of epirubicin from raw product

doi:10.1081/JLC-100106952

	Application of preparative chromatography to the isolation of epirubicin from raw product
	Qi, Y; Huang, JX
	2001
Source Publication	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume	24 Issue:18 Pages:2837-2848
Abstract	Epirubicin (4'-epidoxorubicin) has been found to be useful in the treatment of cancer. The isolation of milligram amounts of epirubicin from raw product was performed by reversed-phase high-performance liquid chromatography (R-P-HPLC) on Kromasil KR100- 10C(18) (250 x 4.6 mm I.D., 10 mum) column. The purity of epirubicin is required to be greater than 99%. First, is adjusting the mobile phase composition, the column temperature, and the flow-rate to increase the separation factor between the desired component and the nearest neighbor. Then, it is possible to increase the column efficiency and to reduce the time of separation required. Besides, displacement effects could be utilized efficiently to increase the sample size and, therefore, the production rate. The location of the cut points for the collection of target compound was determined by use of detector response levels. This method can be used as a guide to scale up the industrially preparative separation of epirubicin.
Department	环境化学与生态毒理学国家重点实验室
Citation statistics
Document Type	期刊论文
Identifier	https://ir.rcees.ac.cn/handle/311016/23994
Collection	环境化学与生态毒理学国家重点实验室
Recommended Citation GB/T 7714	Qi, Y,Huang, JX. Application of preparative chromatography to the isolation of epirubicin from raw product[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2001,24(18):2837-2848.
APA	Qi, Y,&Huang, JX.(2001).Application of preparative chromatography to the isolation of epirubicin from raw product.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,24(18),2837-2848.
MLA	Qi, Y,et al."Application of preparative chromatography to the isolation of epirubicin from raw product".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 24.18(2001):2837-2848.

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