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Effects of nitrogen deposition rates and frequencies on the abundance of soil nitrogen-related functional genes in temperate grassland of northern China
Ning, Qiushi; Gu, Qian; Shen, Jupei; Lv, Xiaotao; Yang, Junjie; Zhang, Ximei; He, Jizheng; Huang, Jianhui; Wang, Hong; Xu, Zhihong; Han, Xingguo
2015-03
Source PublicationJOURNAL OF SOILS AND SEDIMENTS
Volume15Issue:3Pages:694-704
AbstractMicrobial processes driving nitrogen (N) cycling are hot topics in terms of increasing N deposition. Abundances of N-related functional genes (NFG) can be most responsive to N deposition and commonly used to represent N transformation rates. However, empirically simulated N deposition has been exclusively conducted through large and infrequent N fertilization, which may have caused contrasting effects on NFGs. Therefore, experiments with small and frequent N additions closed to natural deposition are necessary. Independently manipulated N addition rates (i.e., 0, 5, 10, 15, 20, and 50 g N m(-2) year(-1)) and two frequencies (2 times per year addition as conventional large and infrequent N fertilization (2 N), and 12 times per year addition simulating small and frequent N deposition (12 N), respectively) were conducted in a long-term field experiment of a semiarid grassland in northern China. Quantification analysis using real-time PCR were carried out for NFGs, including nifH for N fixation, chiA for N mineralization, archaeal (AOA) and bacterial (AOB) amoA for nitrification, and narG, nirS, nirK, and nosZ for denitrification. NFG abundances showed distinct sensitivities to N addition rates. The nifH, AOA-amoA, nirS, and nosZ gene abundances increased due to improved available N at low N rates, but suppressed by salt toxicity and acidification at high N rates. Large changes of chiA and AOB-amoA gene abundances highlighted their great sensitivities to the N enrichment. The abundance of AOB-amoA was more sensitive to N addition than AOA-amoA, but AOA-amoA dominated in absolute numbers and they predominated the ammonia-oxidation under different conditions. The N addition frequencies caused significant lower gene abundances of nifH, nirS, and nosZ under the 2-N frequency due to stronger suppression of acidification and salt toxicity and resulted in significant higher AOB-amoA gene abundances in response to higher N availability under the 2-N frequency. The NFGs abundances responded to N addition rates distinctly, highlighting that the driven processes involved in N cycling were altered by the N addition rates. The different effects of two N addition frequencies on NFG abundances demonstrated that conventional large and infrequent N fertilization cannot represent N deposition, and small and frequent N addition should be employed to project the effects of N deposition on microbial functional groups as well as on N transformations.
Department土壤环境科学实验室
KeywordFunctional Gene Nitrogen Addition Frequency Nitrogen Addition Rate Nitrogen Deposition Nitrogen Transformation Temperate Grassland
WOS Research AreaGeosciences, Multidisciplinary ; Soil Science
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Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/32893
Collection土壤环境科学实验室
Recommended Citation
GB/T 7714
Ning, Qiushi,Gu, Qian,Shen, Jupei,et al. Effects of nitrogen deposition rates and frequencies on the abundance of soil nitrogen-related functional genes in temperate grassland of northern China[J]. JOURNAL OF SOILS AND SEDIMENTS,2015,15(3):694-704.
APA Ning, Qiushi.,Gu, Qian.,Shen, Jupei.,Lv, Xiaotao.,Yang, Junjie.,...&Han, Xingguo.(2015).Effects of nitrogen deposition rates and frequencies on the abundance of soil nitrogen-related functional genes in temperate grassland of northern China.JOURNAL OF SOILS AND SEDIMENTS,15(3),694-704.
MLA Ning, Qiushi,et al."Effects of nitrogen deposition rates and frequencies on the abundance of soil nitrogen-related functional genes in temperate grassland of northern China".JOURNAL OF SOILS AND SEDIMENTS 15.3(2015):694-704.
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