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Simultaneous determination of eight catechins and four theaflavins in green, black and oolong tea using new HPLC-MS-MS method
Tao, Wuqun; Zhou, Zhiguang; Zhao, Bin; Wei, Tongyu
2016-11-30
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume131Issue:0Pages:140-145
AbstractA simple, rapid and accurate analytical method was developed for the analysis of eight tea catechins and four theaflavins simultaneously in three types of tea (green, black and oolong tea), using ultra high performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HPLC-MS-MS) in multiple-reaction monitoring (MRM) mode. This method using HPLC-MS-MS in positive mode was performed on a CAPCELL PAK C18 MGIII (2.0 mm x 100 mm, 3 mu m) column (Shiseido) with the mobile phase consisting of 0.1% aqueous formic acid (A) and methanol (B) in gradient elution ion at a flow rate of 03 mL min(-1), and the column temperature set at 30 degrees C The optimized HPLC-MS-MS methodology is selective and specific, and was validated for eight catechins and four theaflavins widely reported in different teas. Satisfactory linearity was achieved in linear range (0.02-5 mu gmL(-1) for catechins and 0.02-20 mu gmL(-1) for theaflavins) and fine determination coefficient (r(2) > 0.9935). The recoveries ranged from 65% to 115% with the RSD ranging from 2.4% to 6.7%. The methodology was used to evaluate the target polyphenols concentration in three types of tea samples. (C) 2016 Elsevier B.V. All rights reserved.
Department环境化学与生态毒理学国家重点实验室
KeywordCatechins Theaflavins Hplc-ms-ms Green Tea Black Tea Oolong Tea
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Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/35946
Collection环境化学与生态毒理学国家重点实验室
Recommended Citation
GB/T 7714
Tao, Wuqun,Zhou, Zhiguang,Zhao, Bin,et al. Simultaneous determination of eight catechins and four theaflavins in green, black and oolong tea using new HPLC-MS-MS method[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2016,131(0):140-145.
APA Tao, Wuqun,Zhou, Zhiguang,Zhao, Bin,&Wei, Tongyu.(2016).Simultaneous determination of eight catechins and four theaflavins in green, black and oolong tea using new HPLC-MS-MS method.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,131(0),140-145.
MLA Tao, Wuqun,et al."Simultaneous determination of eight catechins and four theaflavins in green, black and oolong tea using new HPLC-MS-MS method".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 131.0(2016):140-145.
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