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Temporal succession of soil antibiotic resistance genes following application of swine, cattle and poultry manures spiked with or without antibiotics
Zhang, Yu-Jing; Hu, Hang-Wei; Gou, Min; Wang, Jun-Tao; Chen, Deli; He, Ji-Zheng
2017-12-01
Source PublicationENVIRONMENTAL POLLUTION
Volume231Issue:0Pages:1621-1632
AbstractLand application of animal manure is a common agricultural practice potentially leading to dispersal and propagation of antibiotic resistance genes (ARGs) in environmental settings. However, the fate of resistome in agro-ecosystems over time following application of different manure sources has never been compared systematically. Here, soil microcosm incubation was conducted to compare effects of poultry, cattle and swine manures spiked with or without the antibiotic tylosin on the temporal changes of soil ARGs. The high-throughput quantitative PCR detected a total of 185 unique ARGs, with Macrolide Lincosamide-Streptogramin B resistance as the most frequently encountered ARG type. The diversity and abundance of ARGs significantly increased following application of manure and manure spiked with tylosin, with more pronounced effects observed in the swine and poultry manure treatments than in the cattle manure treatment. The level of antibiotic resistance gradually decreased over time in all manured soils but was still significantly higher in the soils treated with swine and poultry manures than in the untreated soils after 130 days' incubation. Tylosin-amended soils consistently showed higher abundances of ARGs than soils treated with manure only, suggesting a strong selection pressure of antibiotic spiked manure on soil ARGs. The relative abundance of ARGs had significantly positive correlations with integrase and transposase genes, indicative of horizontal transfer potential of ARGs in manure and tylosin treated soils. Our findings provide evidence that application of swine and poultry manures might enrich more soil ARGs than cattle manure, which necessitates the appropriate treatment of raw animal manures prior to land application to minimise the spread of environmental ARGs. (C) 2017 Elsevier Ltd. All rights reserved.
Department土壤环境科学实验室
KeywordAnimal Manure Tylosin Antibiotic Resistance Genes Mobile Genetic Elements Public Health
Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/39604
Collection土壤环境科学实验室
Recommended Citation
GB/T 7714
Zhang, Yu-Jing,Hu, Hang-Wei,Gou, Min,et al. Temporal succession of soil antibiotic resistance genes following application of swine, cattle and poultry manures spiked with or without antibiotics[J]. ENVIRONMENTAL POLLUTION,2017,231(0):1621-1632.
APA Zhang, Yu-Jing,Hu, Hang-Wei,Gou, Min,Wang, Jun-Tao,Chen, Deli,&He, Ji-Zheng.(2017).Temporal succession of soil antibiotic resistance genes following application of swine, cattle and poultry manures spiked with or without antibiotics.ENVIRONMENTAL POLLUTION,231(0),1621-1632.
MLA Zhang, Yu-Jing,et al."Temporal succession of soil antibiotic resistance genes following application of swine, cattle and poultry manures spiked with or without antibiotics".ENVIRONMENTAL POLLUTION 231.0(2017):1621-1632.
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