RCEES OpenIR  > 环境化学与生态毒理学国家重点实验室
A selective adsorption-based separation of low-mass molecules from biological samples towards high-throughput mass spectrometry analysis in a single drop of human whole blood
Wang, Jing1,3; Liang, Yong1; Mao, Yuxiang3; Liu, Qian1,2,4; Jiang, Guibin2,4
2019-09-01
Source PublicationTALANTA
ISSN0039-9140
Volume202Pages:237-243
AbstractIn this work, a newly designed multifunctional adsorbent material, octadecyl-modified ordered mesoporous carbon (C-18-CMK-8), was synthesized and employed for simultaneous analysis of multiple small molecules by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The C-18-CMK-8 was facilely prepared by one-step chemical binding of octadecyltrimethoxysilane to an ordered mesoporous carbon CMK-8. The nanosized pore structure of C-18-CMK-8 can selectively enrich low-mass molecules while excluding interferences from large molecules in whole blood; meanwhile, the C-18 chain can greatly enhance the affinity for a wide range of small molecules. Then, the C-18-CMK-8-based solid-phase extraction (SPE) combined with MALDI-TOF MS measurement was applied in rapid and high-throughput screening of six doping agents (triamterene, trenbolone, testosterone, methyltestosterone, clenbuterol, and strychnine)) in single-drop human whole blood samples. The method showed high analytical sensitivity (detection limit 0.05-0.1 ng mL(-1)), good recoveries (67.2-114.3%), minimal sample requirement (similar to 20 mu L), and robust anti-interference ability. With distinct advantages such as high throughput, rapidness, minimal sample requirement, and high sensitivity, this study not only offers a versatile enrichment material for complex sample preparation, but also demonstrates a promising tool for minimal whole blood analysis.
Department环境化学与生态毒理学国家重点实验室
KeywordOrdered mesoporous carbon Adsorbent Sample preparation MALDI-TOF MS Mass spectrometry Single-drop whole blood
Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/42499
Collection环境化学与生态毒理学国家重点实验室
Affiliation1.Jianghan Univ, Hubei Key Lab Environm & Hlth Effects Persistent, Wuhan 430056, Hubei, Peoples R China
2.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China
3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
4.Henan Polytech Univ, Inst Resources & Environm, Jiaozuo 454003, Henan, Peoples R China
5.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jing,Liang, Yong,Mao, Yuxiang,et al. A selective adsorption-based separation of low-mass molecules from biological samples towards high-throughput mass spectrometry analysis in a single drop of human whole blood[J]. TALANTA,2019,202:237-243.
APA Wang, Jing,Liang, Yong,Mao, Yuxiang,Liu, Qian,&Jiang, Guibin.(2019).A selective adsorption-based separation of low-mass molecules from biological samples towards high-throughput mass spectrometry analysis in a single drop of human whole blood.TALANTA,202,237-243.
MLA Wang, Jing,et al."A selective adsorption-based separation of low-mass molecules from biological samples towards high-throughput mass spectrometry analysis in a single drop of human whole blood".TALANTA 202(2019):237-243.
Files in This Item: Download All
File Name/Size DocType Version Access License
A selective adsorpti(4136KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Jing]'s Articles
[Liang, Yong]'s Articles
[Mao, Yuxiang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Jing]'s Articles
[Liang, Yong]'s Articles
[Mao, Yuxiang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Jing]'s Articles
[Liang, Yong]'s Articles
[Mao, Yuxiang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A selective adsorption-based separation of low-mass molecules from biological samples towards high-throughput mass spectrometry analysis in a single drop of human whole blood.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.