| Application of electrophysiological technique in toxicological study: From manual to automated patch-clamp recording | |
Gao, Jia; Zhang, He; Xiong, Ping; Yan, Xueting; Liao, Chunyang; Jiang, Guibin
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| 2020-12 | |
| Source Publication | TRAC-TRENDS IN ANALYTICAL CHEMISTRY
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| ISSN | 0165-9936 |
| Volume | 133Pages:1-9 |
| Abstract | Ion channels are common targets of a range of environmental substances, including pharmaceuticals, toxins, metals and many organic pollutants. Patch-clamp technique is gold standard in studying ion channel characteristics, contributing to the improved accuracy in active compound identification, the increasing studies of molecular mechanism behind diseases, and the better prediction of potential toxicants. Nevertheless, low throughput and high technical capability requirement limit the application of conventional manual patch-clamp recording and an emerging transformation towards automation and simplification is rising. The evolving automated patch-clamp technique shows obvious advances over manual technique in many aspects, including reproducibility, throughput, operability and standardization. The application of emerging automated patch-clamp platform has created valuable consequences in many research areas, especially in high throughput screening. In this study, we provided an overview on the progresses made by conventional manual and emerging automated patch-clamp techniques in toxicological studies. (C) 2020 Elsevier B.V. All rights reserved. |
| Department | 环境化学与生态毒理学国家重点实验室 |
| Keyword | Manual patch-clamp Automated patch-clamp Ion channel characterization High throughput screening Toxicity |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/44607 |
| Collection | 环境化学与生态毒理学国家重点实验室 |
| Recommended Citation GB/T 7714 | Gao, Jia,Zhang, He,Xiong, Ping,et al. Application of electrophysiological technique in toxicological study: From manual to automated patch-clamp recording[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2020,133:1-9. |
| APA | Gao, Jia,Zhang, He,Xiong, Ping,Yan, Xueting,Liao, Chunyang,&Jiang, Guibin.(2020).Application of electrophysiological technique in toxicological study: From manual to automated patch-clamp recording.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,133,1-9. |
| MLA | Gao, Jia,et al."Application of electrophysiological technique in toxicological study: From manual to automated patch-clamp recording".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 133(2020):1-9. |
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| File Name/Size | DocType | Version | Access | License | ||
| Application of elect（682KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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