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Immunoassay of Small Molecule Mediated by a Triply Functional DNA
Li, Yapiao; Yu, Fangzhi; Chen, Shaokun; Zhang, Ning; Wang, Hailin; Zhao, Qiang
AbstractBenefiting from specific target recognition by antibodies, the immunoassay is one of the widely used assays for the detection of biologically and environmentally important small molecules in broad fields. It can be challenge to isolate small molecules from their antibody complex in an immobilization-free immunoassay with separation for the detection of small-molecule targets. Here we present an immunoassay mediated by a triply functional DNA probe. A DNA strand is dually labeled with a fluorophore and the target small molecule. This DNA probe integrates three functions, including specific binding to the antibody, signal reporting for sensitive fluorescence detection, and carrying negative charges to facilitate capillary electrophoresis (CE) separation. The binding of the probe to an antibody brings many negative charges in the complex and causes significant changes in mass-to-charge ratios, so the antibody-probe complex can be well separated from the unbound probe in CE analysis. A simple immunoassay is achieved by target competition with this DNA probe for antibody binding in CE coupled to ultrasensitive laser-induced fluorescence (LIF) detection. To show a proof of concept, we detected two model small-molecule targets, digoxin, a therapeutic drug, and ochratoxin A (OTA), an important mycotoxin for food safety. In addition, the use of two DNA probes with distinguished migration times in CE allowed the simultaneous detection of OTA and digoxin. This immunoassay provides new opportunities for wide applications.
WOS Research AreaChemistry, Analytical
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.UCAS, Hangzhou Inst Adv Study, Sch Environm, Hangzhou 310000, Peoples R China
Recommended Citation
GB/T 7714
Li, Yapiao,Yu, Fangzhi,Chen, Shaokun,et al. Immunoassay of Small Molecule Mediated by a Triply Functional DNA[J]. ANALYTICAL CHEMISTRY,2021,93(11):4794-4799.
APA Li, Yapiao,Yu, Fangzhi,Chen, Shaokun,Zhang, Ning,Wang, Hailin,&Zhao, Qiang.(2021).Immunoassay of Small Molecule Mediated by a Triply Functional DNA.ANALYTICAL CHEMISTRY,93(11),4794-4799.
MLA Li, Yapiao,et al."Immunoassay of Small Molecule Mediated by a Triply Functional DNA".ANALYTICAL CHEMISTRY 93.11(2021):4794-4799.
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