| Interaction of mercury ion (Hg2+) with blood and cytotoxicity attenuation by serum albumin binding | |
Song, Shanjun; Li, Yiling; Liu, Qian S.; Wang, Huiyu; Li, Penghui; Shi, Jianbo; Hu, Ligang; Zhang, Haiyan; Liu, Yuanchen; Li, Kun ; Zhao, Xingchen; Cai, Zongwei
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| 2021-06-15 | |
| Source Publication | JOURNAL OF HAZARDOUS MATERIALS
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| ISSN | 0304-3894 |
| Volume | 412Pages:- |
| Abstract | Blood mercury reflects the amount available from tissues, which is an indication of the exposure level. Here we confirm that Hg2+ caused hemolytic effects at high concentrations; while at light concentrations, most of the ions were bound to human serum albumin (HSA). The binding mechanism of Hg2+ to HSA has been investigated, which indicated that the presence of Hg2+ significantly perturbed the structure of HSA and quenched the fluorescence of protein in a hybrid dynamic and static mode. Hg2+ was preferably bound to cysteine and cystine, where the R-S-S-R structure is responsible for maintaining the protein?s structure by stabilizing the ?-helical bundles. The metal-protein interaction mitigated the cellular toxicity as concealed by A498 cell lines. The fundamental and comprehensive data in this work is beneficial to elucidating and understanding the identification and binding mechanisms of heavy metals with proteins, as well as possible risks on human beings and the environment. |
| Department | 环境化学与生态毒理学国家重点实验室 |
| Keyword | Mercury Plasma Protein identification Binding mechanism Reduced toxicity |
| WOS Research Area | Engineering, Environmental ; Environmental Sciences |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/45761 |
| Collection | 环境化学与生态毒理学国家重点实验室 |
| Affiliation | 1.Natl Inst Metrol, Beijing 100013, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 3.Tianjin Univ Technol, Tianjin 300384, Peoples R China 4.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China 5.Hong Kong Baptist Univ, Dept Chem, State Key Lab Environm & Biol Anal, Hong Kong, Peoples R China 6.Henan Univ, Sch Life Sci, State Key Lab Cotton Biol, Key Lab Plant Stress Biol, Kaifeng |
| Recommended Citation GB/T 7714 | Song, Shanjun,Li, Yiling,Liu, Qian S.,et al. Interaction of mercury ion (Hg2+) with blood and cytotoxicity attenuation by serum albumin binding[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,412:-. |
| APA | Song, Shanjun.,Li, Yiling.,Liu, Qian S..,Wang, Huiyu.,Li, Penghui.,...&Cai, Zongwei.(2021).Interaction of mercury ion (Hg2+) with blood and cytotoxicity attenuation by serum albumin binding.JOURNAL OF HAZARDOUS MATERIALS,412,-. |
| MLA | Song, Shanjun,et al."Interaction of mercury ion (Hg2+) with blood and cytotoxicity attenuation by serum albumin binding".JOURNAL OF HAZARDOUS MATERIALS 412(2021):-. |
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| Interaction of mercu（3845KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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