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Probing the Formation Kinetics and Thermodynamics with Rationally Designed Analytical Tools Enables One-Pot Synthesis and Purification of a Tetrahedral DNA Nanostructure
Gao, Lu; Liu, Liying; Tian, Yunfei; Yang, Qianfan; Wu, Peng; Fan, Chunhai; Zhao, Qiang; Li, Feng
2021-05-11
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume93Issue:18Pages:7045-7053
AbstractThe development of robust analytical tools capable of probing the formation kinetics and thermodynamics of DNA nanostructures is a crucial step toward better understanding and manufacturing of diverse DNA-based materials. Herein, we introduce a real-time fluorescence anisotropy assay and rationally designed DNA reaction termination probes (DRTPs) as a set of new tools for exploring the formation mechanisms of DNA nanostructures. We deployed these tools for probing the formation of a classic tetrahedral DNA nanostructure (TDN) as a model system. Our tools revealed that the formation of TDN was dominated by simultaneous hybridization, whereas its undesired side products were caused mainly through step-wise hybridization. An optimal reaction temperature exists that favors the formation of TDN over side products. With insight into the TDN formation mechanism, we further engineered magnetic DRTPs to achieve single-step purification of TDN, enabling 10-fold improvement in the ratio between the targeted TDN and undesired side products without tedious procedures or bulky instruments. Combining the optimal reaction and purification conditions, we finally demonstrated the one-pot synthesis and purification of TDN. The analytical techniques offered in this work may hold potential to find wide applications and inspire new analytical methods for structural DNA nanotechnology.
Department环境化学与生态毒理学国家重点实验室
WOS Research AreaChemistry, Analytical
Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/45821
Collection环境化学与生态毒理学国家重点实验室
Affiliation1.Sichuan Univ, Coll Chem, Analyt & Testing Ctr, Key Lab Green Chem & Technol,Minist Educ, Chengdu 610064, Sichuan, Peoples R China
2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 201240, Peoples R China
5.Brock Univ, Ctr Biotechnol, Dept Chem, St Catharines, ON L2S 3A1, Canada
Recommended Citation
GB/T 7714
Gao, Lu,Liu, Liying,Tian, Yunfei,et al. Probing the Formation Kinetics and Thermodynamics with Rationally Designed Analytical Tools Enables One-Pot Synthesis and Purification of a Tetrahedral DNA Nanostructure[J]. ANALYTICAL CHEMISTRY,2021,93(18):7045-7053.
APA Gao, Lu.,Liu, Liying.,Tian, Yunfei.,Yang, Qianfan.,Wu, Peng.,...&Li, Feng.(2021).Probing the Formation Kinetics and Thermodynamics with Rationally Designed Analytical Tools Enables One-Pot Synthesis and Purification of a Tetrahedral DNA Nanostructure.ANALYTICAL CHEMISTRY,93(18),7045-7053.
MLA Gao, Lu,et al."Probing the Formation Kinetics and Thermodynamics with Rationally Designed Analytical Tools Enables One-Pot Synthesis and Purification of a Tetrahedral DNA Nanostructure".ANALYTICAL CHEMISTRY 93.18(2021):7045-7053.
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