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The cell-impermeable Ru(II) polypyridyl complex as a potent intracellular photosensitizer under visible light irradiation via ion-pairing with suitable lipophilic counter-anions
Yan, Zhu-Ying; Chen, Jing; Shao, Jie; Jiao, Ze-Qing; Tang, Tian-Shu; Tang, Miao; Sheng, Zhi-Guo; Mao, Li; Huang, Rong; Huang, Chun-Hua; Zhang, Zhi-Hui; Su, Hong-Mei; Zhu, Ben-Zhan
2021-08-01
Source PublicationFREE RADICAL BIOLOGY AND MEDICINE
ISSN0891-5849
Volume171Pages:69-79
AbstractDeveloping the cell-impermeable Ru(II) polypyridyl cationic complexes as effective photosensitizers (PS) which have high cellular uptake and photo-toxicity, but low dark toxicity, is quite challenging. Here we found that the highly reactive singlet oxygen (1O2) can be generated by the irradiation of a typical Ru(II) polypyridyl complex Ru(II)tris(tetramethylphenanthroline) ([Ru(TMP)3]2+) under visible light irradiation by ESR with TEMPO (2,2,6,6-tetramethyl-4-piperidone-N-oxyl) as 1O2 probe. Effective cellular and nuclear delivery of cationic [Ru (TMP)3]2+ was achieved through our recently developed ion-pairing method, and 2,3,4,5-tetrachlorophenol (2,3,4,5-TeCP) was found to be the most effective among all chlorophenols tested. The accelerated cellular, especially nuclear uptake of [Ru(TMP)3]2+ results in the formation of 8-oxo-7,8-dihydro-2 '-deoxyguanosine (8oxodG) and DNA strand breaks, caspase 3/7 activation and cell apoptosis in HeLa cells upon light irradiation. More importantly, compared with other traditional photosensitizers, [Ru(TMP)3]2+ showed significant phototoxicity but low dark toxicity. Similar effects were observed when 2,3,4,5-TeCP was substituted by the currently clinically used anti-inflammatory drug flufenamic acid. This represents the first report that the cellimpermeable Ru(II) polypyridyl complex ion-paired with suitable lipophilic counter-anions functions as potent intracellular photosensitizer under visible light irradiation mainly via a 1O2-mediated mechanism. These findings should provide new perspectives for future investigations on other metal complexes with similar characteristics as promising photosensitizers for potential photodynamic therapy.
Department环境化学与生态毒理学国家重点实验室
KeywordRu(II) polypyridyl complex Photosensitizer Singlet oxygen Cellular uptake Ion-pairing Photodynamic therapy
WOS Research AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism
Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/45855
Collection环境化学与生态毒理学国家重点实验室
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China
4.Chongqing Univ, Coll Bioengn, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400044, Peoples R China
5.Peking Univ Third Hosp, Dept Stomatol, Beijing 100191, Peoples R China
6.Res Ctr Ecoenvironm Sci, Joint Inst Environm Sci, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Yan, Zhu-Ying,Chen, Jing,Shao, Jie,et al. The cell-impermeable Ru(II) polypyridyl complex as a potent intracellular photosensitizer under visible light irradiation via ion-pairing with suitable lipophilic counter-anions[J]. FREE RADICAL BIOLOGY AND MEDICINE,2021,171:69-79.
APA Yan, Zhu-Ying.,Chen, Jing.,Shao, Jie.,Jiao, Ze-Qing.,Tang, Tian-Shu.,...&Zhu, Ben-Zhan.(2021).The cell-impermeable Ru(II) polypyridyl complex as a potent intracellular photosensitizer under visible light irradiation via ion-pairing with suitable lipophilic counter-anions.FREE RADICAL BIOLOGY AND MEDICINE,171,69-79.
MLA Yan, Zhu-Ying,et al."The cell-impermeable Ru(II) polypyridyl complex as a potent intracellular photosensitizer under visible light irradiation via ion-pairing with suitable lipophilic counter-anions".FREE RADICAL BIOLOGY AND MEDICINE 171(2021):69-79.
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