| In situ High-Throughput Single-Cell Analysis Reveals the Crosstalk between Nanoparticle-Induced Cell Responses | |
Wang, Yuanyuan; Wang, Fengbang; Chen, Zihan; Song, Maoyong ; Yao, Xinglei; Jiang, Guibin
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| 2021-04-20 | |
| Source Publication | ENVIRONMENTAL SCIENCE & TECHNOLOGY
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| ISSN | 0013-936X |
| Volume | 55Issue:8Pages:5136-5142 |
| Abstract | Nanomaterials are widely used in a variety of industrial, biological, and medical applications. Therefore, high concerns about their possible impact on human and environmental health have been raised. Here, we describe a highthroughput single-cell imaging method to reveal the crosstalk among quantum dot (QDot)-induced ROS generation, apoptosis, and changes in nucleus size in macrophages. In triple marker combinations, we assessed the correlations of three QDot-induced cellular responses via divided subsets based on single-cell analysis. In contrast to the results obtained from the cell population, we demonstrated that the change in nucleus size was positively correlated with ROS generation. We found that QDot exposure induced ROS generation, which led to cell apoptosis, followed by a change in nucleus size. In general, these observations on crosstalk of cellular responses provide detailed insights into the heterogeneity of nanoparticle exposure. |
| Department | 环境纳米技术与健康效应重点实验室 |
| WOS Research Area | Engineering, Environmental ; Environmental Sciences |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/45888 |
| Collection | 环境纳米技术与健康效应重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Environm Nanotechnol & Hlth Effects, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Yuanyuan,Wang, Fengbang,Chen, Zihan,et al. In situ High-Throughput Single-Cell Analysis Reveals the Crosstalk between Nanoparticle-Induced Cell Responses[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2021,55(8):5136-5142. |
| APA | Wang, Yuanyuan,Wang, Fengbang,Chen, Zihan,Song, Maoyong,Yao, Xinglei,&Jiang, Guibin.(2021).In situ High-Throughput Single-Cell Analysis Reveals the Crosstalk between Nanoparticle-Induced Cell Responses.ENVIRONMENTAL SCIENCE & TECHNOLOGY,55(8),5136-5142. |
| MLA | Wang, Yuanyuan,et al."In situ High-Throughput Single-Cell Analysis Reveals the Crosstalk between Nanoparticle-Induced Cell Responses".ENVIRONMENTAL SCIENCE & TECHNOLOGY 55.8(2021):5136-5142. |
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| In situ High-Through（4139KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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