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In situ High-Throughput Single-Cell Analysis Reveals the Crosstalk between Nanoparticle-Induced Cell Responses
Wang, Yuanyuan; Wang, Fengbang; Chen, Zihan; Song, Maoyong; Yao, Xinglei; Jiang, Guibin
2021-04-20
Source PublicationENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN0013-936X
Volume55Issue:8Pages:5136-5142
AbstractNanomaterials are widely used in a variety of industrial, biological, and medical applications. Therefore, high concerns about their possible impact on human and environmental health have been raised. Here, we describe a highthroughput single-cell imaging method to reveal the crosstalk among quantum dot (QDot)-induced ROS generation, apoptosis, and changes in nucleus size in macrophages. In triple marker combinations, we assessed the correlations of three QDot-induced cellular responses via divided subsets based on single-cell analysis. In contrast to the results obtained from the cell population, we demonstrated that the change in nucleus size was positively correlated with ROS generation. We found that QDot exposure induced ROS generation, which led to cell apoptosis, followed by a change in nucleus size. In general, these observations on crosstalk of cellular responses provide detailed insights into the heterogeneity of nanoparticle exposure.
Department环境纳米技术与健康效应重点实验室
WOS Research AreaEngineering, Environmental ; Environmental Sciences
Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/45888
Collection环境纳米技术与健康效应重点实验室
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Environm Nanotechnol & Hlth Effects, Beijing 100085, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yuanyuan,Wang, Fengbang,Chen, Zihan,et al. In situ High-Throughput Single-Cell Analysis Reveals the Crosstalk between Nanoparticle-Induced Cell Responses[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2021,55(8):5136-5142.
APA Wang, Yuanyuan,Wang, Fengbang,Chen, Zihan,Song, Maoyong,Yao, Xinglei,&Jiang, Guibin.(2021).In situ High-Throughput Single-Cell Analysis Reveals the Crosstalk between Nanoparticle-Induced Cell Responses.ENVIRONMENTAL SCIENCE & TECHNOLOGY,55(8),5136-5142.
MLA Wang, Yuanyuan,et al."In situ High-Throughput Single-Cell Analysis Reveals the Crosstalk between Nanoparticle-Induced Cell Responses".ENVIRONMENTAL SCIENCE & TECHNOLOGY 55.8(2021):5136-5142.
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