KMS of Research Center for Eco-Environmental Sciences， CAS
| Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in waters via surface enhanced Raman scattering (SERS) | |
Zhang, Dayi; Zhang, Xiaoling; Ma, Rui; Deng, Songqiang; Wang, Xinzi; Wang, Xinquan; Zhang, Xian; Huang, Xia; Liu, Yi; Li, Guanghe; Qu, Jiuhui ; Zhu, Yu; Li, Junyi
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| 2021-07-15 | |
| Source Publication | WATER RESEARCH
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| ISSN | 0043-1354 |
| Volume | 200Pages:- |
| Abstract | The outbreak of coronavirus infectious disease-2019 (COVID-19) pneumonia challenges the rapid interrogation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human and environmental samples. In this study, we developed an assay using surface enhanced Raman scattering (SERS) coupled with multivariate analysis to detect SARS-CoV-2 in an ultra-fast manner without any pretreatment (e.g., RNA extraction). Using silver-nanorod SERS array functionalized with cellular receptor angiotensin-converting enzyme 2 (ACE2), we obtained strong SERS signals of ACE2 at 1032, 1051, 1089, 1189, 1447 and 1527 cm(-1). The recognition and binding of receptor binding domain (RBD) of SARS-CoV-2 spike protein on SERS assay significantly quenched the spectral intensities of most peaks and exhibited a shift from 1189 to 1182 cm(-1). On-site tests on 23 water samples with a portable Raman spectrometer proved its accuracy and easy-operation for spot detection of SARS-CoV-2 to evaluate disinfection performance, explore viral survival in environmental media, assess viral decay in wastewater treatment plant and track SARS-CoV-2 in pipe network. Our findings raise a state-of-the-art spectroscopic tool to screen and interrogate viruses with RBD for human cell entry, proving its feasibility and potential as an ultra-fast detection tool for wastewater-based epidemiology. (C) 2021 Elsevier Ltd. All rights reserved. |
| Department | 中国科学院饮用水科学与技术重点实验室 |
| Keyword | SARS-CoV-2 Surface enhanced Raman scattering (SERS) Human receptor angiotensin converting enzyme 2 (ACE2) Wastewater-based epidemiology |
| WOS Research Area | Engineering, Environmental ; Environmental Sciences ; Water Resources |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/46272 |
| Collection | 中国科学院饮用水科学与技术重点实验室 |
| Affiliation | 1.Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China 2.Suzhou Yiqing Environm Sci & Technol LTD, Suzhou 215163, Peoples R China 3.Res Inst Environm Innovat Tsinghua Suzhou, Suzhou 215163, Peoples R China 4.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China 5.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China 6.Chinese Acad Sci, Suzhou Inst Nanotech & Nanob, 398 Ruoshui Rd,Suzhou Ind Pk, Suzhou 215 |
| Recommended Citation GB/T 7714 | Zhang, Dayi,Zhang, Xiaoling,Ma, Rui,et al. Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in waters via surface enhanced Raman scattering (SERS)[J]. WATER RESEARCH,2021,200:-. |
| APA | Zhang, Dayi.,Zhang, Xiaoling.,Ma, Rui.,Deng, Songqiang.,Wang, Xinzi.,...&Li, Junyi.(2021).Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in waters via surface enhanced Raman scattering (SERS).WATER RESEARCH,200,-. |
| MLA | Zhang, Dayi,et al."Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in waters via surface enhanced Raman scattering (SERS)".WATER RESEARCH 200(2021):-. |
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| Ultra-fast and onsit（2107KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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