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Stochastic processes regulate belowground community assembly in alpine grasslands on the Tibetan Plateau
Kang, Luyao; Chen, Leiyi; Zhang, Dianye; Peng, Yunfeng; Song, Yutong; Kou, Dan; Deng, Ye; Yang, Yuanhe
2021-11-09
Source PublicationENVIRONMENTAL MICROBIOLOGY
ISSN0269-7491
Volume291Pages:-
AbstractMicrobial-assisted antimonate [Sb(V)] reduction immobilizes this redox-sensitive metalloid in the subsurface. Most indigenous aerobes in antimony (Sb)-contaminated areas do not contain Sb(V)-reducing genes but can resist high levels of Sb(V) threat. Herein, to unravel the mechanisms of Sb(V) resistance by aerobes, we used Escherichia coli W3110 as a model aerobe and incubated it with 10 mu M Sb(V). We found that strain W3110, without known Sb(V)-reducing genes, was able to reduce Sb(V) to Sb(III). Our transcriptome analysis and reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) results show that the Sb(V) threat at the 10 mu M level had a negligible effect on the gene expression of strain W3110. In vitro incubation experiments further indicate that Sb (V) reduction was attributable to extracellular polymeric substances (EPS). Moreover, the three-dimensional excitation-emission matrix fluorescence spectroscopy reveals that the tryptophan-like components in EPS were involved in Sb(V) binding as evidenced by its weakened fluorescence intensity upon Sb(V) addition. The FTIR and XPS analyses indicate that hemiacetal and amide groups in EPS contributed to the reduction of Sb(V). Preculture with 10 mu M Sb(V) did not exhibit a significant difference in Sb(V)-reducing capacity, suggesting that Sb(V) stress probably did not stimulate EPS secretion of W3110. Our results highlight the importance of EPS as the first line of defense against toxins, especially for those bacteria without such functional genes.
Department中国科学院环境生物技术重点实验室
KeywordAntimony Escherichia coli W3110 Extracellular polymeric substances Antimonate reduction Resistance strategy
Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/46900
Collection中国科学院环境生物技术重点实验室
Affiliation1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, CAS Key Lab Environm Biotechnol, Beijing 100085, Peoples R China
Recommended Citation
GB/T 7714
Kang, Luyao,Chen, Leiyi,Zhang, Dianye,et al. Stochastic processes regulate belowground community assembly in alpine grasslands on the Tibetan Plateau[J]. ENVIRONMENTAL MICROBIOLOGY,2021,291:-.
APA Kang, Luyao.,Chen, Leiyi.,Zhang, Dianye.,Peng, Yunfeng.,Song, Yutong.,...&Yang, Yuanhe.(2021).Stochastic processes regulate belowground community assembly in alpine grasslands on the Tibetan Plateau.ENVIRONMENTAL MICROBIOLOGY,291,-.
MLA Kang, Luyao,et al."Stochastic processes regulate belowground community assembly in alpine grasslands on the Tibetan Plateau".ENVIRONMENTAL MICROBIOLOGY 291(2021):-.
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